Dong Hao, He Hui, Wang Min
College of Marine Life Sciences, Key Laboratory of Evolution & Marine Biodiversity (Ministry of Education) and Institute of Evolution & Marine Biodiversity, Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266003, China.
Haide College, Ocean University of China, Qingdao 266100, China.
Microorganisms. 2025 May 12;13(5):1112. doi: 10.3390/microorganisms13051112.
Nitrite oxidation, a pivotal process in the nitrogen cycle driven by microorganisms, is primarily carried out by nitrite-oxidizing bacteria (NOB). While extensive studies on have been conducted in terrestrial habitats, knowledge of in marine sediments remains limited. Therefore, we employed high-throughput sequencing analysis to systematically explore the community structure, assembly processes and potential interactions of in the eastern China marginal seas. Our results exhibit pronounced spatial heterogeneity in communities across seas. Widespread distribution of taxa was observed, with lineage II emerging as the most important group in this study. Based on the neutral community model (NCM), normalized stochasticity ratio (NST) and beta nearest-taxon-index (βNTI) analysis, deterministic processes predominantly shaped the community assembly of . Complex interspecies interactions among were observed with molecular ecological network analysis, and the community in the East China Sea showed the highest complexity, while the community displayed the greatest stability in the South Yellow Sea. In addition, the communities were notably influenced by geographic distances and environmental factors, including salinity, temperature, dissolved oxygen concentration and dissolved inorganic nutrient concentration. These results may expand our understanding of in marine environments and enhance our insights into the marine nitrogen cycle.
亚硝酸盐氧化是由微生物驱动的氮循环中的一个关键过程，主要由亚硝酸盐氧化细菌（NOB）进行。虽然在陆地生境中已经对其进行了广泛研究，但关于海洋沉积物中亚硝酸盐氧化的知识仍然有限。因此，我们采用高通量测序分析，系统地探索中国东部边缘海中亚硝酸盐氧化细菌的群落结构、组装过程及潜在相互作用。我们的结果显示，不同海域的亚硝酸盐氧化细菌群落存在明显的空间异质性。观察到亚硝酸盐氧化细菌类群广泛分布，其中第二谱系在本研究中成为最重要的类群。基于中性群落模型（NCM）、标准化随机性比率（NST）和β最近分类单元指数（βNTI）分析，确定性过程在很大程度上塑造了亚硝酸盐氧化细菌的群落组装。通过分子生态网络分析观察到亚硝酸盐氧化细菌之间存在复杂的种间相互作用，东海的群落表现出最高的复杂性，而南黄海的群落表现出最大的稳定性。此外，亚硝酸盐氧化细菌群落明显受到地理距离和环境因素的影响，包括盐度、温度、溶解氧浓度和溶解无机营养物浓度。这些结果可能会扩展我们对海洋环境中亚硝酸盐氧化细菌的理解，并增强我们对海洋氮循环的认识。
