Jiang Zhitong, Chen Xue, Lei Jinhui, Xue Huizhen, Zhang Bo, Xu Xiaofan, Geng Huijing, Li Zhoukun, Yan Xin, Dong Weiliang, Cao Hui, Cui Zhongli
Key Laboratory of Agricultural Environment Microbiology of the Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.
State Key Laboratory of Material Chemical Engineering, College of Biology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211899, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2023 May 25;39(5):1963-1975. doi: 10.13345/j.cjb.220976.
Polyurethane (PUR) plastics is widely used because of its unique physical and chemical properties. However, unreasonable disposal of the vast amount of used PUR plastics has caused serious environmental pollution. The efficient degradation and utilization of used PUR plastics by means of microorganisms has become one of the current research hotspots, and efficient PUR degrading microbes are the key to the biological treatment of PUR plastics. In this study, an Impranil DLN-degrading bacteria G-11 was isolated from used PUR plastic samples collected from landfill, and its PUR-degrading characteristics were studied. Strain G-11 was identified as sp. through 16S rRNA gene sequence alignment. PUR degradation experiment showed that the weight loss rate of the commercial PUR plastics upon treatment of strain G-11 was 4.67%. Scanning electron microscope (SEM) showed that the surface structure of G-11-treated PUR plastics was destroyed with an eroded morphology. Contact angle and thermogravimetry analysis (TGA) showed that the hydrophilicity of PUR plastics increased along with decreased thermal stability upon treatment by strain G-11, which were consistent with the weight loss and morphological observation. These results indicated that strain G-11 isolated from landfill has potential application in biodegradation of waste PUR plastics.
聚氨酯（PUR）塑料因其独特的物理化学性质而被广泛使用。然而，大量废旧PUR塑料的不合理处置已造成严重的环境污染。利用微生物对废旧PUR塑料进行高效降解和利用已成为当前的研究热点之一，高效的PUR降解微生物是PUR塑料生物处理的关键。在本研究中，从垃圾填埋场采集的废旧PUR塑料样品中分离出一株能降解Impranil DLN的细菌G-11，并对其PUR降解特性进行了研究。通过16S rRNA基因序列比对，菌株G-11被鉴定为 属 种。PUR降解实验表明，经菌株G-11处理后，商用PUR塑料的失重率为4.67%。扫描电子显微镜（SEM）显示，经G-11处理的PUR塑料表面结构被破坏，呈现出侵蚀形态。接触角和热重分析（TGA）表明，经菌株G-11处理后，PUR塑料的亲水性增加，热稳定性降低，这与失重和形态观察结果一致。这些结果表明，从垃圾填埋场分离出的菌株G-11在废旧PUR塑料的生物降解方面具有潜在应用价值。
