Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy.
Methods Mol Biol. 2023;2655:117-124. doi: 10.1007/978-1-0716-3143-0_10.
Replication of distinct genomic loci occurs at different times during cell cycle. The replication timing correlates with chromatin status, three-dimensional folding, and transcriptional potential of the genes. In particular, active genes tend to replicate early in S phase, whereas inactive replicate late. In embryonic stem cells, some early replicating genes are not yet transcribed reflecting their potential to be transcribed upon differentiation. Here, I describe a method for evaluating the proportion of gene loci that is replicated in different phases of cell cycle thus reflecting the replication timing.
不同的基因组位点在细胞周期的不同时间进行复制。复制时间与染色质状态、三维折叠和基因的转录潜力相关。特别是，活性基因往往在 S 期早期复制，而非活性基因则在晚期复制。在胚胎干细胞中，一些早期复制的基因尚未转录，反映了它们在分化时转录的潜力。在这里，我描述了一种评估基因座在细胞周期不同阶段复制比例的方法，从而反映复制时间。
