Department of gynecology, the second people's Hospital affiliated to Fujian University of Traditional Chinese Medicine, China.
WuDepartment of gynecology, the second people's Hospital affiliated to Fujian University of Traditional Chinese Medicine, China.
Cell Mol Biol (Noisy-le-grand). 2023 Jan 31;69(1):109-113. doi: 10.14715/cmb/2022.69.1.19.
Cervical cancer is the fourth most prevalent cancer for females with 14,100 new cases each year globally. Efficient screening and intervention at the precancerous stage is the key point to the prevention and treatment of cervical cancer. However, no widely recognized biomarkers have been discovered yet. We investigated the expression of miR-10b in cervical cells and its correlation with clinicopathological features in different pathological grades of cervical precancerous lesions. The expression of miR-10b in cervical cytology samples from 20 cases of LSIL, 22 cases of HSIL, 18 cases of early-stage cervical cancer, and 20 cases of cervicitis controls were assessed using qPCR. From the same cervical cytology samples, the human papillomavirus (HPV) load was assessed using semi-PCR and the lesion size, and gland involvement levels from the same subjects were assessed during the cervical examination. The correlation between miR-10b expression and different pathological grades of cervical lesions was analyzed. We also calculated the correlation between HPV load, lesion size, gland involvement, P16 expression, and different pathological grades. The expression of miR-10b exhibited a step-decreasing manner from cervicitis control (4.23(4.00,4.71)) to LSIL (2.67(2.52,2.90)), HSIL (1.49(1.30,1.80)) and cervical cancer group (0.65(0.55,0.80)). There is a significant difference (P<0.001) between cervicitis and HSIL, cervicitis and cervical cancer, ISIL and HSIL, as well as ISIL and cervical cancer but not between the cervicitis group and the LSIL group. In addition, more severe pathological grades were correlated with a bigger rate of gland involvement (P＜0.001). We also found that different pathological grades were correlated with the intensity of P16 expression (P=0.001), and the intensity of P16 expression is positively correlated with different pathological grades (P<0.05). Repressed expression of miR-10b is related to the progression of cervical precancerous lesions. Increased gland involvement rate and increased intensity of P16 expression are risk factors for developing cervical cancers. Our result showed that miR-10b may be a potential biomarker for the screening and ranking of cervical precancerous lesions.
宫颈癌是女性第四大常见癌症，全球每年新增病例约 14100 例。在癌前阶段进行有效的筛查和干预是预防和治疗宫颈癌的关键。然而，目前尚未发现广泛认可的生物标志物。我们研究了 miR-10b 在宫颈细胞中的表达及其与不同病理分级宫颈前病变临床病理特征的相关性。采用 qPCR 检测 20 例 LSIL、22 例 HSIL、18 例早期宫颈癌和 20 例宫颈炎对照患者宫颈细胞学样本中 miR-10b 的表达。采用半 PCR 法检测同一宫颈细胞学样本中的人乳头瘤病毒（HPV）负荷，在宫颈检查中评估同一患者的病变大小和腺体受累程度。分析 miR-10b 表达与宫颈病变不同病理分级的相关性。我们还计算了 HPV 负荷、病变大小、腺体受累、P16 表达与不同病理分级的相关性。miR-10b 的表达呈逐渐下降趋势，从宫颈炎对照组（4.23（4.00，4.71））到 LSIL（2.67（2.52，2.90））、HSIL（1.49（1.30，1.80））和宫颈癌组（0.65（0.55，0.80））。宫颈炎与 HSIL、宫颈炎与宫颈癌、LSIL 与 HSIL 以及 LSIL 与宫颈癌之间存在显著差异（P<0.001），但宫颈炎组与 LSIL 组之间无差异。此外，更严重的病理分级与更高的腺体受累率相关（P＜0.001）。我们还发现，不同的病理分级与 P16 表达强度相关（P=0.001），并且 P16 表达强度与不同的病理分级呈正相关（P<0.05）。miR-10b 的抑制表达与宫颈前病变的进展有关。腺体受累率增加和 P16 表达强度增加是宫颈癌发生的危险因素。我们的结果表明，miR-10b 可能是宫颈前病变筛查和分级的潜在生物标志物。
