Baumeister R G, Richter W O, Riel K A, Schwandt P, Bohmert H
Handchir Mikrochir Plast Chir. 1986 May;18(3):115-7.
Subcutaneous adipose tissue was obtained from ten obese patients. Isolated fat cells were incubated with porcine beta-lipotropin but no stimulation of lipolysis was obtained. To avoid accumulation of lipolysis inhibiting substances, such as free fatty acids and adenosin, as well as protease activity, perifusion of isolated fat cells was carried out. Under these test conditions, which allow continuous determination of released glycerol, lipolytic activity could be shown for the peptide hormone beta-lipotropin in human adipocytes.
从十名肥胖患者身上获取皮下脂肪组织。将分离出的脂肪细胞与猪β-促脂解素一起孵育，但未获得脂解刺激。为避免脂解抑制物质（如游离脂肪酸和腺苷）的积累以及蛋白酶活性，对分离出的脂肪细胞进行了灌流。在这些能够连续测定释放甘油的测试条件下，可显示出肽激素β-促脂解素在人脂肪细胞中的脂解活性。
