用于疾病严重程度预测的唾液和鼻咽拭子样本中的新型冠状病毒2型逆转录定量聚合酶链反应（SARS-CoV-2 RT-qPCR）循环阈值（Ct）值
SARS-CoV-2 RT-qPCR Ct values in saliva and nasopharyngeal swab samples for disease severity prediction.
作者信息
Snipaitiene Kristina, Zablockiene Birute, Sabaliauskaite Rasa, Zukauskaite Kristina, Matulyte Elzbieta, Smalinskaite Tautvile, Paulauskas Mindaugas, Zablockis Rolandas, Lopeta Mantvydas, Gagilas Julius, Puriene Alina, Jancoriene Ligita, Jarmalaite Sonata
机构信息
Institute of Biomedical Sciences, Life Sciences Center, Vilnius University, Vilnius, Lithuania.
Laboratory of Genetic Diagnostic, National Cancer Institute of Lithuania, Vilnius, Lithuania.
出版信息
J Oral Microbiol. 2023 May 17;15(1):2213106. doi: 10.1080/20002297.2023.2213106. eCollection 2023.
BACKGROUND
Comparison of clinical value of RT-qPCR-based SARS-CoV-2 tests performed on saliva samples (SSs) and nasopharyngeal swab samples (NPSs) for prediction of the COVID-19 disease severity.
METHODS
Three paired SSs and NPSs collected every 3 days from 100 hospitalised COVID-19 patients during 2020 Jul-2021 Jan were tested by RT-qPCR for the original SARS-CoV-2 virus and compared to 150 healthy controls. Cases were divided into mild+moderate (Cohort I, = 47) and severe disease (Cohort II, = 53) cohorts and compared.
RESULTS
SARS-CoV-2 was detected in 65% (91/140) vs. 53% (82/156) of NPSs and 49% (68/139) vs. 48% (75/157) of SSs collected from Cohort I and II, respectively, resulting in the total respective detection rates of 58% (173/296) vs. 48% (143/296) ( = 0.017). Ct values of SSs were lower than those of NPSs (mean Ct = 28.01 vs. 30.07, = 0.002). Although Ct values of the first SSs were significantly lower in Cohort I than in Cohort II ( = 0.04), it became negative earlier (mean 11.7 vs. 14.8 days, = 0.005). Multivariate Cox proportional hazards regression analysis showed that Ct value ≤30 from SSs was the independent predictor for severe COVID-19 (HR = 10.06, 95% CI: 1.84-55.14, = 0.008).
CONCLUSION
Salivary RT-qPCR testing is suitable for SARS-CoV-2 infection control, while simple measurement of Ct values can assist in prediction of COVID-19 severity.
背景
比较基于逆转录定量聚合酶链反应（RT-qPCR）对唾液样本（SSs）和鼻咽拭子样本（NPSs）进行的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）检测在预测2019冠状病毒病（COVID-19）疾病严重程度方面的临床价值。
方法
2020年7月至2021年1月期间，从100名住院的COVID-19患者中每3天收集一次的3对SSs和NPSs，通过RT-qPCR检测原始SARS-CoV-2病毒，并与150名健康对照进行比较。病例分为轻度+中度（队列I，n = 47）和重度疾病（队列II，n = 53）队列并进行比较。
结果
分别从队列I和队列II收集的NPSs中，SARS-CoV-2的检测率为65%（91/140）对53%（82/156），SSs中为49%（68/139）对48%（75/157），总检测率分别为58%（173/296）对48%（143/296）（P = 0.017）。SSs的Ct值低于NPSs（平均Ct = 28.01对30.07，P = 0.002）。尽管队列I中首次采集的SSs的Ct值显著低于队列II（P = 0.04），但其转阴时间更早（平均11.7天对14.8天，P = 0.005）。多变量Cox比例风险回归分析显示，SSs的Ct值≤30是重度COVID-19的独立预测因素（风险比[HR] = 10.06，95%置信区间[CI]：1.84 - 55.14，P = 0.008）。
结论
唾液RT-qPCR检测适用于SARS-CoV-2感染控制，而简单测量Ct值有助于预测COVID-19的严重程度。
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