Rao L G, Wang H M, Kalliecharan R, Heersche J N, Sodek J
Histochem J. 1979 Jan;11(1):73-82. doi: 10.1007/BF01041266.
Antibody against Type I collagen was raised in rabbits and purified by immunoadsorption on Sepharose-conjugated Types I and III collagen. The cross-reactivity of purified antibody to Type III collagen was found to be less than 0.5% by passive haemagglutination and less than 1.5% by radioimmunoassay. When paraffin sections of fixed and decalcified pig molars were incubated with purified antibody to Type I collagen, varying degrees of staining were observed in the ligament, gingiva, bone and cementum. The periodontal ligament adjacent to bone was more widely stained than that adjacent to cementum in some regions, whereas in others, no difference in staining could be discerned between the two halves of the ligament. The lamina propria of gingiva was stained, and this appeared to be most intense in the vicinity of the overlying epithelium. The fibrous component in the endosteal spaces, the dentine and the extracellular coronal elements in the pulp were generally stained. The impression obtained from the staining pattern is that Type I collagen is not restricted to particular regions of the periodontal ligament or the lamina propria of the gingiva.
抗I型胶原蛋白抗体在兔体内产生，并通过在琼脂糖偶联的I型和III型胶原蛋白上进行免疫吸附来纯化。通过被动血凝试验发现，纯化抗体与III型胶原蛋白的交叉反应性小于0.5%，通过放射免疫测定法小于1.5%。当用纯化的抗I型胶原蛋白抗体孵育固定和脱钙猪磨牙的石蜡切片时，在韧带、牙龈、骨和牙骨质中观察到不同程度的染色。在某些区域，与骨相邻的牙周韧带染色比与牙骨质相邻的更广泛，而在其他区域，韧带两半之间的染色没有差异。牙龈固有层被染色，并且在上覆上皮附近似乎最为强烈。骨内膜间隙中的纤维成分、牙本质和牙髓中的细胞外冠部成分通常被染色。从染色模式得到的印象是，I型胶原蛋白不限于牙周韧带或牙龈固有层的特定区域。
