Singh G, Singh P K
Int J Dermatol. 1986 Jun;25(5):324-6. doi: 10.1111/j.1365-4362.1986.tb02258.x.
Ten healthy volunteers were studied to find out whether tachyphylaxis occurs on repeated application of topical steroids. The Singh and Singh modification of the Reddy and Singh (1976) technique was used. Fluocinolone acetonide cream (0.1%) under occlusion was applied daily for 14 days on the flexor aspect of the forearms. The wheal was induced by pricking with histamine acid phosphate solution and the volume of the wheal produced was calculated. The test was repeated on the same sites on alternate days for 14 days. The maximum wheal suppression, which gradually diminished, was observed on the 8th day. By the 14th day, there was practically total tolerance to topical steroid, which showed minimal suppression of histamine wheal formation. This confirms the occurrence of the phenomenon of tachyphylaxis by different experimental techniques.
对10名健康志愿者进行了研究，以确定局部类固醇反复应用时是否会发生快速耐受现象。采用了Reddy和Singh（1976年）技术的Singh和Singh改良法。将0.1%的醋酸氟轻松乳膏在封闭条件下每日涂于前臂屈侧，持续14天。用磷酸组胺溶液针刺诱发风团，并计算所产生风团的体积。在14天内每隔一天在相同部位重复进行该试验。在第8天观察到最大风团抑制作用逐渐减弱。到第14天，对局部类固醇几乎完全耐受，组胺风团形成的抑制作用极小。这通过不同的实验技术证实了快速耐受现象的发生。
