School of Earth Sciences and Environmental Engineering, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.
Research Center for Innovative Energy and Carbon Optimized Synthesis for Chemicals (inn-ECOSysChem), Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea.
J Microbiol Biotechnol. 2023 Aug 28;33(8):1084-1090. doi: 10.4014/jmb.2304.04011. Epub 2023 May 18.
The strain KIST612, initially identified as , was a suspected member of due to differences in phenotype, genotype, and average nucleotide identity (ANI). Here, we found that ATCC 8486 and KIST612 are genetically different in their central metabolic pathways, such as that of carbon metabolism. Although 16S rDNA sequencing of KIST612 revealed high identity with ATCC 8486 (99.2%) and DSM 3662 (99.8%), phylogenetic analysis of housekeeping genes and genome metrics clearly indicated that KIST612 belongs to . The phylogenies showed that KIST612 is closer to DSM 3662 than to ATCC 8486. The ANI between KIST612 and DSM 3662 was 99.8%, which was above the species cut-off of 96%, Meanwhile, the ANI value with ATCC 8486 was not significant, showing only 94.6%. The digital DNA-DNA hybridization (dDDH) results also supported the ANI values. The dDDH between KIST612 and DSM 3662 was 98.4%, whereas between KIST612 and ATCC 8486, it was 57.8%, which is lower than the species cut-off of 70%. Based on these findings, we propose the reclassification of KIST612 as KIST612.
最初被鉴定为 的 KIST612 菌株，由于表型、基因型和平均核苷酸同一性 (ANI) 的差异，被怀疑是 的一个成员。在这里，我们发现 ATCC 8486 和 KIST612 在其中心代谢途径（如碳代谢）方面存在遗传差异。尽管 KIST612 的 16S rDNA 测序与 ATCC 8486（99.2%）和 DSM 3662（99.8%）高度一致，但看家基因和基因组指标的系统发育分析清楚地表明 KIST612 属于 。系统发育分析表明，KIST612 与 DSM 3662 的亲缘关系比与 ATCC 8486 更近。KIST612 与 DSM 3662 之间的 ANI 为 99.8%，高于 96%的种间界限，而与 ATCC 8486 的 ANI 值不显著，仅为 94.6%。数字 DNA-DNA 杂交（dDDH）结果也支持了 ANI 值。KIST612 与 DSM 3662 之间的 dDDH 为 98.4%，而 KIST612 与 ATCC 8486 之间的 dDDH 为 57.8%，低于 70%的种间界限。基于这些发现，我们建议将 KIST612 重新分类为 KIST612。
