Vergel-Suarez Ariadna H, García-Martínez Janet B, López-Barrera Germán L, Barajas-Solano Andrés F, Zuorro Antonio
Department of Environmental Sciences, Universidad Francisco de Paula Santander, Av. Gran Colombia No. 12E-96, Cúcuta 540003, Colombia.
Department of Chemical Engineering, Materials, and Environment, Sapienza University, Via Eudossiana 18, 00184 Roma, Italy.
BioTech (Basel). 2023 Apr 23;12(2):30. doi: 10.3390/biotech12020030.
Drying the biomass produced is one of the critical steps to avoid cell degradation; however, its high energy cost is a significant technological barrier to improving this type of bioprocess's technical and economic feasibility. This work explores the impact of the biomass drying method of a strain of sp. on the extraction efficiency of a phycoerythrin-rich protein extract. To achieve the above, the effect of time (12-24 h), temperature (40-70 °C), and drying method (convection oven and dehydrator) were determined using an I-best design with a response surface. According to the statistical results, the factors that most influence the extraction and purity of phycoerythrin are temperature and moisture removal by dehydration. The latter demonstrates that gentle drying of the biomass allows removing the most significant amount of moisture from the biomass without affecting the concentration or quality of temperature-sensitive proteins.
干燥所产生的生物质是避免细胞降解的关键步骤之一;然而,其高昂的能源成本是提高此类生物工艺技术和经济可行性的重大技术障碍。这项工作探讨了一种 菌株的生物质干燥方法对富含藻红蛋白的蛋白质提取物提取效率的影响。为实现上述目标,使用具有响应面的I-最优设计确定了时间(12 - 24小时)、温度(40 - 70°C)和干燥方法(对流烘箱和脱水机)的影响。根据统计结果,对藻红蛋白提取和纯度影响最大的因素是温度和脱水去除水分。后者表明,对生物质进行温和干燥可以从生物质中去除最多的水分,而不会影响温度敏感型蛋白质的浓度或质量。