Vergel-Suarez Ariadna H, García-Martínez Janet B, López-Barrera German L, Urbina-Suarez Néstor A, Barajas-Solano Andrés F
Department of Environmental Sciences, Universidad Francisco de Paula Santander, Av. Gran Colombia No. 12E-96, Cúcuta 540003, Colombia.
BioTech (Basel). 2024 Jun 24;13(3):21. doi: 10.3390/biotech13030021.
This work aimed to identify the influence of pH, molarity, / fraction, extraction time, agitation, and either a sodium (NaHPO·7HO-NaHPO·HO) or potassium buffer (KHPO-KHPO) used in the extraction of C-phycoerythrin (C-PE) from a thermotolerant strain of sp. An experimental design (Minimum Run Resolution V Factorial Design) and a Central Composite Design (CCD) were used. According to the statistical results of the first design, the K-PO buffer, pH, molarity, and / fraction are vital factors that enhance the extractability of C-PE. The construction of a CCD design of the experiments suggests that the potassium phosphate buffer at pH 5.8, longer extraction times (50 min), and minimal extraction speed (1000 rpm) are ideal for maximizing C-PE concentration, while purity is unaffected by the design conditions. This optimization improves extraction yields and maintains the desired bright purple color of the phycobiliprotein.
这项工作旨在确定pH值、摩尔浓度、/ 分数、提取时间、搅拌以及用于从嗜热 菌株中提取C-藻红蛋白（C-PE）的钠（NaHPO·7H₂O - NaHPO·H₂O）或钾缓冲液（KH₂PO₄ - K₂HPO₄）的影响。采用了实验设计（最小运行分辨率V因子设计）和中心复合设计（CCD）。根据第一个设计的统计结果，K-PO₄缓冲液、pH值、摩尔浓度和 / 分数是提高C-PE可提取性的关键因素。实验的CCD设计表明，pH 5.8的磷酸钾缓冲液、较长的提取时间（50分钟）和最小提取速度（1000转/分钟）是使C-PE浓度最大化的理想条件，而纯度不受设计条件的影响。这种优化提高了提取产量，并保持了藻胆蛋白所需的亮紫色。
