Influence of Critical Parameters on the Extraction of Concentrated C-PE from Thermotolerant Cyanobacteria.

This work aimed to identify the influence of pH, molarity, / fraction, extraction time, agitation, and either a sodium (NaHPO·7HO-NaHPO·HO) or potassium buffer (KHPO-KHPO) used in the extraction of C-phycoerythrin (C-PE) from a thermotolerant strain of sp. An experimental design (Minimum Run Resolution V Factorial Design) and a Central Composite Design (CCD) were used. According to the statistical results of the first design, the K-PO buffer, pH, molarity, and / fraction are vital factors that enhance the extractability of C-PE. The construction of a CCD design of the experiments suggests that the potassium phosphate buffer at pH 5.8, longer extraction times (50 min), and minimal extraction speed (1000 rpm) are ideal for maximizing C-PE concentration, while purity is unaffected by the design conditions. This optimization improves extraction yields and maintains the desired bright purple color of the phycobiliprotein.