State Key Laboratory of Separation Membranes and Membrane Processes, School of Material Science and Engineering, Tiangong University, Tianjin, 300387, China.
State Key Laboratory of Separation Membranes and Membrane Processes, School of Chemistry, Tiangong University, Tianjin 300387, China.
J Mater Chem B. 2023 Jun 7;11(22):5000-5009. doi: 10.1039/d3tb00687e.
Glucose-sensitive membranes have promising applications in insulin release. Phenylboronic acid (PBA) is an important glucose reporter. Most of PBA-based glucose-sensitive materials are expansion-type, which cannot act as chemical valves in porous membranes for self-regulated insulin release. In this study, a glucose-sensitive membrane with PBA-based contraction-type amphiphilic block copolymer polystyrene--poly(-isopropylacrylamide--2-(acrylamido) phenylboronic acid) (PSNB) as chemical valves was constructed through non-solvent induced phase separation (NIPS) method. Due to surface segregation, hydrophobic polystyrene (PS) component can anchor in the membrane matrix to improve the stability of the membrane, and glucose-sensitive hydrophilic poly(-isopropylacrylamide--2-(acrylamido) phenylboronic acid) (PNB) component can expose on the surfaces of the membrane and the channels to provide glucose-sensitivity of the membrane. With increasing the polymer content or chain length of the hydrophilic component, the glucose sensitivity of the membrane was improved. The blend membrane showed glucose-sensitive insulin release behavior in simulated body fluids (SBF) and fetal bovine serum (FBS). The membrane also exhibited good antifouling properties and biocompatibility.
葡萄糖敏感膜在胰岛素释放方面具有广阔的应用前景。苯硼酸（PBA）是一种重要的葡萄糖报告分子。大多数基于 PBA 的葡萄糖敏感材料为膨胀型，无法在多孔膜中作为化学阀用于自我调节胰岛素释放。在本研究中，通过非溶剂致相分离（NIPS）法构建了一种以基于 PBA 的缩合型两亲嵌段共聚物聚苯乙烯-聚（-异丙基丙烯酰胺-2-（丙烯酰胺基）苯硼酸）（PSNB）为化学阀的葡萄糖敏感膜。由于表面选择性富集，疏水性聚苯乙烯（PS）组分可以锚定在膜基质中，从而提高膜的稳定性，而葡萄糖敏感的亲水性聚（-异丙基丙烯酰胺-2-（丙烯酰胺基）苯硼酸）（PNB）组分则可以暴露在膜表面和通道上，赋予膜葡萄糖敏感性。随着亲水组分的聚合物含量或链长的增加，膜的葡萄糖敏感性得到提高。该共混膜在模拟体液（SBF）和胎牛血清（FBS）中表现出葡萄糖敏感的胰岛素释放行为。该膜还表现出良好的抗污染性能和生物相容性。
