Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Biotecnología, Inmunología y Genética. Catedra de Genética Forense, Centro de Referencia en Identificación Humana y Servicio de Huellas Digitales Genéticas (SHDG), Universidad de Buenos Aires, CABA, Argentina.
CONICET. Consejo Nacional de Investigaciones Científicas y Técnicas, CABA, Argentina.
Mol Biol Rep. 2023 Jul;50(7):6287-6291. doi: 10.1007/s11033-023-08497-z. Epub 2023 May 23.
Argentinean population is the result of admixture between South Amerindians, Europeans and to a lesser degree, Africans. Since the advent of forensic molecular genetics, the construction of local reference databases became mandatory. Aiming to further extend the technical quality reference database of Argentina, we present herein the allele frequencies for 24 autosomal STRs, including D22S1045, and SE33 (not previously reported for Argentina in STRidER).
Genotypes of 6454 unrelated individuals (3761 males and 2694 females) from 13 out of 23 provinces were analysed. Forensic parameters were calculated for each marker. The observed heterozygosity ranged from 0.661 (TPOX) to 0.941 (SE33). The locus SE33 was revealed to be the most informative marker showing the highest values for PIC (0.955), GD (0.952), TPI (8.455) and PE (0.879). On the other hand, TPOX turned out to be the least informative marker: PIC (0.618), GD (0.669), and PE (0.371). The high number of analyzed individuals allowed detecting low frequency alleles and microvariants in CSF1PO; D16S539 and D21S11 D18S51; PENTA D; PENTA E and at locus D6S1043.
This study is the most extensive for Argentina and complements the already reported information concerning the autosomal STRs commonly used in forensic identification. The results were submitted passing STRidER quality control standards (QC), receiving the reference number STR000327 v.2.
阿根廷人口是南印第安人、欧洲人和在较小程度上的非洲人混合的结果。自从法医分子遗传学出现以来，建立当地参考数据库已成为强制性要求。为了进一步扩展阿根廷技术质量参考数据库，我们在此介绍了 24 个常染色体 STR 的等位基因频率，包括 D22S1045 和 SE33（此前在 STRidER 中未报告过阿根廷的 SE33）。
分析了来自阿根廷 23 个省份中的 13 个省份的 6454 名无关个体（3761 名男性和 2694 名女性）的基因型。为每个标记计算了法医参数。观察到的杂合度范围从 0.661（TPOX）到 0.941（SE33）。SE33 基因座被证明是最具信息量的标记，其 PIC（0.955）、GD（0.952）、TPI（8.455）和 PE（0.879）值最高。另一方面，TPOX 是信息量最低的标记：PIC（0.618）、GD（0.669）和 PE（0.371）。分析的个体数量众多，使得 CSF1PO 中的低频等位基因和微变体、D16S539 和 D21S11、D18S51、PENTA D、PENTA E 和 D6S1043 基因座能够被检测到。
这是阿根廷最广泛的研究，补充了已报告的有关法医鉴定中常用的常染色体 STR 的信息。结果通过 STRidER 质量控制标准（QC）提交，获得参考编号 STR000327 v.2。
