Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.
Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Nigeria.
Vet Ital. 2022 Dec 31;58(3). doi: 10.12834/VetIt.2295.15822.2.
African swine fever (ASF) is a contagious viral disease that affects pigs of all ages, inducing hemorrhagic fever with high mortality and severe threat to pig production. This study investigated the hematological and serum biochemical abnormalities associated with a natural ASF infection in pigs. A total of 100 serum samples of pigs from piggery suspected of ASFV infection were screened for antibodies by ELISA. Thirty‑two blood samples from serologically positive pigs and 32 negative pigs were undergo to hematological and serum biochemical analyses following standard procedures. The results showed that the mean values of the red blood cell (RBC) count, total white blood cell (TWBC) count, absolute lymphocyte count, absolute monocyte count, serum total protein (TP) and globulin were significantly (p < 0.05) lower while the mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), absolute neutrophil count and serum gamma glutamyl transferase (GGT) were significantly (p < 0.05) higher in the infected than the healthy pigs. There were no significant differences (p > 0.05) in the mean values of the packed cell volume (PCV), hemoglobin concentration, absolute eosinophil count, cholesterol, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities between the infected and healthy pigs. Hence, natural ASFV infection may have caused alterations in the hematological and serum biochemical parameters in the infected pigs. The generated data could complement the existing laboratory diagnostic techniques such as polymerase chain reaction, direct fluorescence antibody test, indirect fluorescent antibody test and ELISA in the diagnosis of ASF in pigs.
非洲猪瘟（ASF）是一种传染性病毒性疾病，影响所有年龄段的猪，引起出血性高热，死亡率高，对养猪业构成严重威胁。本研究调查了与猪自然感染 ASF 相关的血液学和血清生化异常。采用 ELISA 法对来自疑似 ASF 感染猪场的 100 份猪血清样本进行抗体筛查。对 32 份血清学阳性猪和 32 份血清学阴性猪的 32 份血液样本进行了血液学和血清生化分析，操作符合标准程序。结果表明，与健康猪相比，感染猪的红细胞（RBC）计数、总白细胞（TWBC）计数、绝对淋巴细胞计数、绝对单核细胞计数、血清总蛋白（TP）和球蛋白的平均值显著（p < 0.05）降低，而平均红细胞体积（MCV）、平均红细胞血红蛋白浓度（MCHC）、绝对中性粒细胞计数和血清γ-谷氨酰转移酶（GGT）的平均值显著（p < 0.05）升高。感染猪与健康猪的红细胞压积（PCV）、血红蛋白浓度、绝对嗜酸性粒细胞计数、胆固醇、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性的平均值无显著差异（p > 0.05）。因此，自然 ASFV 感染可能导致感染猪的血液学和血清生化参数发生变化。所产生的数据可以补充现有的实验室诊断技术，如聚合酶链反应、直接荧光抗体试验、间接荧光抗体试验和 ELISA，用于猪 ASF 的诊断。
