MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Laboratory of Flexible Electronics Technology, Center for Synthetic and Systems Biology, Tsinghua University, Beijing, China.
TUS-Pharmaceutical Group Co, Ltd, Hengyang, China.
Expert Opin Drug Discov. 2023 Jul;18(7):781-795. doi: 10.1080/17460441.2023.2216013. Epub 2023 May 23.
High content screening (HCS) is an important tool for drug screening. However, the potential of HCS in the field of drug screening and synthetic biology is limited by traditional culture platforms that use multi-well plates, which have several disadvantages. Recently, microfluidic devices have gradually been applied in HCS, which significantly reduces experimental costs, increases assay throughput, and improves the accuracy of drug screening.
This review provides an overview of microfluidic devices for high-content screening in drug discovery platforms, including droplet, microarray, and organs-on-chip technologies.
HCS is a promising technology increasingly adopted by the pharmaceutical industry as well as academic researchers for drug discovery and screening. In particular, microfluidic-based HCS shows unique advantages, and microfluidics technology has promoted significant advancements and broader usage and applicability of HCS in drug discovery. With the integration of stem cell, gene editing technology, and other biological technologies, microfluidics-based HCS will expand the application scope of personalized disease and drug screening models. The authors anticipate rapid developments in this field, with microfluidic-based approaches becoming increasingly important in HCS applications.
高通量筛选（HCS）是药物筛选的重要工具。然而，传统的基于多孔板的培养平台限制了 HCS 在药物筛选和合成生物学领域的应用，因为这些平台存在一些缺点。最近，微流控芯片已逐渐应用于 HCS 中，这显著降低了实验成本，提高了检测通量，并提高了药物筛选的准确性。
本文综述了用于药物发现平台的高通量筛选的微流控芯片，包括液滴、微阵列和芯片上器官技术。
HCS 是一项很有前途的技术，越来越多的制药公司和学术研究人员将其应用于药物发现和筛选。特别是基于微流控的 HCS 显示出独特的优势，微流控技术促进了 HCS 在药物发现中的显著发展以及更广泛的应用和适用性。随着干细胞、基因编辑技术和其他生物技术的整合，基于微流控的 HCS 将扩展个性化疾病和药物筛选模型的应用范围。作者预计该领域将快速发展，基于微流控的方法将在 HCS 应用中变得越来越重要。
