Pei Hongcui, Li Yushan, Liu Yanhong, Liu Pan, Zhang Jialin, Ren Xueni, Lu Zefu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
aBIOTECH. 2023 Feb 10;4(1):8-19. doi: 10.1007/s42994-023-00095-8. eCollection 2023 Mar.
Development of wheat ( L) grain mainly depends on the processes of starch synthesis and storage protein accumulation, which are critical for grain yield and quality. However, the regulatory network underlying the transcriptional and physiological changes of grain development is still not clear. Here, we combined ATAC-seq and RNA-seq to discover the chromatin accessibility and gene expression dynamics during these processes. We found that the chromatin accessibility changes are tightly associated with differential transcriptomic expressions, and the proportion of distal ACRs was increased gradually during grain development. Specific transcription factor (TF) binding sites were enriched at different stages and were diversified among the 3 subgenomes. We further predicted the potential interactions between key TFs and genes related with starch and storage protein biosynthesis and found different copies of some key TFs played diversified roles. Overall, our findings have provided numerous resources and illustrated the regulatory network during wheat grain development, which would shed light on the improvement of wheat yields and qualities.
The online version contains supplementary material available at 10.1007/s42994-023-00095-8.
小麦（L）籽粒的发育主要依赖于淀粉合成和贮藏蛋白积累过程，这对籽粒产量和品质至关重要。然而，籽粒发育过程中转录和生理变化的调控网络仍不清楚。在这里，我们结合ATAC-seq和RNA-seq来发现这些过程中的染色质可及性和基因表达动态。我们发现染色质可及性变化与差异转录组表达紧密相关，并且在籽粒发育过程中远端ACR的比例逐渐增加。特定转录因子（TF）结合位点在不同阶段富集，并且在3个亚基因组之间存在差异。我们进一步预测了关键TF与淀粉和贮藏蛋白生物合成相关基因之间的潜在相互作用，发现一些关键TF的不同拷贝发挥了不同的作用。总体而言，我们的研究结果提供了大量资源，并阐明了小麦籽粒发育过程中的调控网络，这将为提高小麦产量和品质提供线索。
在线版本包含可在10.1007/s42994-023-00095-8获取的补充材料。
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