College of Agronomy, Anhui Agricultural University, Hefei, 230036, Anhui, China.
Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow and Huai River Valley, Ministry of Agriculture, Hefei, 230036, China.
Planta. 2022 May 14;255(6):125. doi: 10.1007/s00425-022-03893-4.
More methylation changes occur in late interval than in early interval of wheat seed development with protein and the starch synthesis-related pathway enriched in the later stages. Wheat seed development is a critical process to determining wheat yield and quality, which is controlled by genetics, epigenetics and environments. The N6-methyladenosine (mA) modification is a reversible and dynamic process and plays regulatory role in plant development and stress responses. To better understand the role of mA in wheat grain development, we characterized the mA modification at 10 day post-anthesis (DPA), 20 DPA and 30 DPA in wheat grain development. mA-seq identified 30,615, 30,326, 27,676 high confidence mA peaks from the 10DPA, 20DPA, and 30DPA, respectively, and enriched at 3'UTR. There were 29,964, 29,542 and 26,834 unique peaks identified in AN0942_10d, AN0942_20d and AN0942_30d. One hundred and forty-two genes were methylated by mA throughout seed development, 940 genes methylated in early grain development (AN0942_20d vs AN0942_10d), 1542 genes in late grain development (AN0942_30d vs AN0942_20d), and 1190 genes between early and late development stage (AN0942_30d vs AN0942_10d). KEGG enrichment analysis found that protein-related pathways and the starch synthesis-related pathway were significantly enriched in the later stages of seed development. Our results provide novel knowledge on mA dynamic changes and its roles in wheat grain development.
在小麦种子发育的晚期间隔期发生的甲基化变化比早期间隔期多，蛋白质和淀粉合成相关途径在后期富集。小麦种子发育是决定小麦产量和品质的关键过程，受遗传、表观遗传和环境控制。N6-甲基腺嘌呤（mA）修饰是一个可逆和动态的过程，在植物发育和应激反应中发挥调节作用。为了更好地理解 mA 在小麦籽粒发育中的作用，我们在小麦籽粒发育的 10 天授粉后（DPA）、20 DPA 和 30 DPA 三个时期对 mA 修饰进行了特征分析。mA-seq 分别从 10DPA、20DPA 和 30DPA 中鉴定出 30615、30326 和 27676 个高置信 mA 峰，富集在 3'UTR。在 AN0942_10d、AN0942_20d 和 AN0942_30d 中分别鉴定出 29964、29542 和 26834 个独特峰。142 个基因在整个种子发育过程中被 mA 甲基化，940 个基因在早期籽粒发育中被甲基化（AN0942_20d 与 AN0942_10d），1542 个基因在晚期籽粒发育中被甲基化（AN0942_30d 与 AN0942_20d），1190 个基因在早期和晚期发育阶段之间被甲基化（AN0942_30d 与 AN0942_10d）。KEGG 富集分析发现，蛋白质相关途径和淀粉合成相关途径在种子发育的后期显著富集。我们的研究结果为 mA 动态变化及其在小麦籽粒发育中的作用提供了新的知识。
