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采用改良AOAC方法II从生花生中提取黄曲霉毒素时的最佳甲醇浓度及溶剂/花生比例

Optimum methanol concentration and solvent/peanut ratio for extraction of aflatoxin from raw peanuts by modified AOAC method II.

作者信息

Whitaker T B, Dickens J W, Giesbrecht F G

出版信息

J Assoc Off Anal Chem. 1986 May-Jun;69(3):508-10.

PMID:3722102
Abstract

The amount of aflatoxin extracted from raw peanuts by using the water-slurry modification of AOAC Method II was determined for 49 different combinations of methanol concentrations and solvent/peanut ratio. Results indicate that the amount of aflatoxins B1 and B2 extracted from raw peanuts is a function of both methanol concentration and solvent/peanut ratio, and a cubic equation was developed, using regression techniques, to describe the combined effects. From the functional relationship, the predicted methanol concentration and solvent/peanut ratio that extracts the most aflatoxin B1 was computed to be 60.0% and 10.8 mL solvent/g peanuts, respectively. This combination extracted 12.1% more aflatoxin than did AOAC Method II.

摘要

采用美国官方分析化学师协会(AOAC)方法II的水浆改良法,测定了从49种不同甲醇浓度和溶剂/花生比例组合的生花生中提取的黄曲霉毒素量。结果表明,从生花生中提取的黄曲霉毒素B1和B2的量是甲醇浓度和溶剂/花生比例两者的函数,并且利用回归技术建立了一个三次方程来描述其综合效应。根据函数关系,计算得出提取最多黄曲霉毒素B1的预测甲醇浓度和溶剂/花生比例分别为60.0%和10.8 mL溶剂/g花生。与AOAC方法II相比,这种组合提取的黄曲霉毒素多12.1%。

相似文献

1
Optimum methanol concentration and solvent/peanut ratio for extraction of aflatoxin from raw peanuts by modified AOAC method II.采用改良AOAC方法II从生花生中提取黄曲霉毒素时的最佳甲醇浓度及溶剂/花生比例
J Assoc Off Anal Chem. 1986 May-Jun;69(3):508-10.
2
Effects of methanol concentration and solvent:peanut ratio on extraction of aflatoxin from raw peanuts.甲醇浓度和溶剂与花生比例对从生花生中提取黄曲霉毒素的影响。
J Assoc Off Anal Chem. 1984 Jan-Feb;67(1):35-6.
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Dilution errors in aflatoxin determinations caused by compounds extracted from peanuts.花生中提取的化合物导致黄曲霉毒素测定中的稀释误差。
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Evaluation of enzyme-linked immunosorbent assay of cleanup for thin-layer chromatography of aflatoxin B1 in corn, peanuts, and peanut butter.玉米、花生和花生酱中黄曲霉毒素B1薄层色谱法净化的酶联免疫吸附测定评估。
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Minicolumn detection methods for aflatoxin in raw peanuts: collaborative study.生花生中黄曲霉毒素的微柱检测方法:协作研究
J Assoc Off Anal Chem. 1981 May;64(3):674-7.
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Solvent-efficient thin-layer chromatographic method for the determination of aflatoxins B1, B2, G1, and G2 in corn and peanut products: collaborative study.用于测定玉米和花生制品中黄曲霉毒素B1、B2、G1和G2的高效溶剂薄层色谱法:协同研究
J AOAC Int. 1994 May-Jun;77(3):637-46.
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J Assoc Off Anal Chem. 1979 Nov;62(6):1281-4.
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Visual and semiquantitative spectrophotometric ELISA screening method for aflatoxin B1 in corn and peanut products: follow-up collaborative study.玉米和花生制品中黄曲霉毒素B1的可视化和半定量分光光度酶联免疫吸附测定筛查方法:后续协同研究
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Rapid determination of aflatoxins in raw peanuts by liquid chromatography with postcolumn iodination and modified minicolumn cleanup.采用柱后碘化和改良微柱净化的液相色谱法快速测定生花生中的黄曲霉毒素。
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Improved liquid chromatographic method for determination of aflatoxins in peanut butter and other commodities.用于测定花生酱及其他商品中黄曲霉毒素的改进液相色谱法。
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