胃癌中的 Epstein-Barr 病毒与潜伏膜蛋白 1 30 bp 缺失多态性的关联。

Epstein-Barr virus in gastric cancer and association with 30 bp del-latent membrane protein 1 polymorphism.

机构信息

Universidade Federal do Rio de Janeiro, Hospital Universitário Clementino Fraga Filho - Rio de Janeiro (RJ), Brazil.

Universidade Federal do Rio de Janeiro, Faculdade de Medicina, Departamento de Patologia - Rio de Janeiro (RJ), Brazil.

出版信息

Rev Assoc Med Bras (1992). 2023 May 19;69(5):e20221571. doi: 10.1590/1806-9282.20221571. eCollection 2023.

DOI:10.1590/1806-9282.20221571

PMID:37222327

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10204858/

Abstract

OBJECTIVE

This study aimed to determine the frequencies of Epstein-Barr virus, types 1 and 2 infection, and 30 bp del-latent membrane protein 1 viral polymorphism in gastric adenocarcinomas, as well as to investigate the association between Epstein-Barr virus infection and tumor location, type, and the patient's sex.

METHODS

Samples were collected from 38 patients treated at a university hospital in Rio de Janeiro, Brazil. Epstein-Barr virus detection and genotyping were performed by polymerase chain reaction, followed by polyacrylamide gel electrophoresis and staining by the silver nitrate method.

RESULTS

Overall, 68.4% of patients had Epstein-Barr virus-positive tumors. Of these, 65.4% presented infection by Epstein-Barr virus type 1, 23.1% by Epstein-Barr virus type 2, and 11.5% had coinfection with types 1 and 2. The 30 bp del-latent membrane protein 1 polymorphism was found in 42.3% of Epstein-Barr virus-positive tumors, 23.1% had the wild-type virus, and 23.1% had the wild-type and the polymorphism concomitantly. In 11.5% of Epstein-Barr virus-positive tumors, it was impossible to determine whether there was polymorphism or not. Tumor location in the antrum (22 of 38) and diffuse type (27 of 38) were predominant. There was no significant difference in Epstein-Barr virus infection or the 30 bp del-latent membrane protein 1 polymorphism between men and women.

CONCLUSION

Epstein-Barr virus infection was found in 68.4% of tumors investigated in this study. To the best of our knowledge, this is the first article showing the coinfection of Epstein-Barr virus types 1 and 2 in gastric carcinoma in Brazil.

摘要

目的

本研究旨在确定胃腺癌中 Epstein-Barr 病毒 1 型和 2 型感染以及 30bp 缺失潜伏膜蛋白 1 病毒多态性的频率，并探讨 Epstein-Barr 病毒感染与肿瘤位置、类型以及患者性别之间的关系。

方法

本研究收集了巴西里约热内卢一所大学医院治疗的 38 名患者的样本。通过聚合酶链反应（PCR）检测 Epstein-Barr 病毒并进行基因分型，随后通过聚丙烯酰胺凝胶电泳和硝酸银染色进行分析。

结果

总体而言，68.4%的患者肿瘤呈 Epstein-Barr 病毒阳性。其中，65.4%为 Epstein-Barr 病毒 1 型感染，23.1%为 Epstein-Barr 病毒 2 型感染，11.5%为两种病毒的混合感染。在 Epstein-Barr 病毒阳性肿瘤中，30bp 缺失潜伏膜蛋白 1 多态性的发生率为 42.3%，野生型病毒为 23.1%，同时存在野生型和多态性的为 23.1%。在 11.5%的 Epstein-Barr 病毒阳性肿瘤中，无法确定是否存在多态性。肿瘤位于胃窦（38 例中的 22 例）和弥漫型（38 例中的 27 例）较为常见。男性和女性之间 Epstein-Barr 病毒感染或 30bp 缺失潜伏膜蛋白 1 多态性没有显著差异。