Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA.
Escola Paulista de Medicina, Federal University of São Paulo, São Paulo, Brazil.
Antimicrob Agents Chemother. 2023 Jun 15;67(6):e0042323. doi: 10.1128/aac.00423-23. Epub 2023 May 24.
We determined the echinocandin susceptibility and genotypes of 13 clinical isolates of Candida auris that were recovered from 4 patients at a tertiary care center in Salvador, Brazil. Three isolates were categorized as echinocandin-resistant, and they harbored a novel mutation that led to an amino acid change W691L located downstream from hot spot 1. When introduced to echinocandin-susceptible C. auris strains by CRISPR/Cas9, Fks1 W691L induced elevated MIC values to all echinocandins (anidulafungin, 16 to 32×; caspofungin, >64×; micafungin, >64×).
我们从巴西萨尔瓦多一家三级保健中心的 4 名患者身上分离出 13 株临床分离的耳念珠菌，确定了它们对棘白菌素的敏感性和基因型。3 株被归类为棘白菌素耐药，它们携带一种新的突变，导致位于热点 1 下游的氨基酸改变 W691L。当通过 CRISPR/Cas9 引入到对棘白菌素敏感的耳念珠菌菌株中时，Fks1 W691L 导致所有棘白菌素（安尼卡fungin，16 至 32×；卡泊芬净，>64×；米卡芬净，>64×）的 MIC 值升高。
