Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, Jiangsu, China.
National Observation and Research Station of Fujian Wuyishan Forest Ecosystem, Wuyishan, 354300, Fujian, China.
Microb Ecol. 2023 Nov;86(4):2330-2343. doi: 10.1007/s00248-023-02240-8. Epub 2023 May 24.
The surface water is an important habitat for freshwater microorganisms, but there is a lack of understanding of the pattern of microbial diversity and structure in stream continuums of small subtropical forest watersheds. Therefore, this study aimed to understand the variations in microbial diversity and community structure along stream orders (1-5) in the small subtropical forest catchments of the Wuyi Mountains. Using GIS software, 20 streams were chosen and classified into 5 orders. Illumina sequencing was used to analyze the dynamics of microbial communities, along with stream orders and hydro-chemical properties of stream water were also determined. Our results indicated that the bacterial and fungal richness (ACE index) was higher in low-order (1 and 2 orders) streams than in high-order (3, 4, and 5 orders) streams, with the highest value in the order 2 streams (P < 0.05). The water temperature and dissolved oxygen were positively correlated with fungal richness (P < 0.05). The bacterial rare taxa had a significant correlation with the abundance taxa (P < 0.05). The relative abundances of Bacteroidetes, Actinobacteria, and Chytridiomycota microbial phyla were significantly different among different order streams (P < 0.05). Using the neutral community model, we found that the fungal community structure was significantly shaped by hydro-chemical properties, while the bacterial community structure was largely regulated by stochastic processes. Our findings suggest that variations in microbial community structure in subtropical headwaters are largely shaped by the water temperature and dissolved oxygen.
地表水是淡水微生物的重要栖息地，但对于小亚热带森林流域溪流连续体中微生物多样性和结构模式的了解还很缺乏。因此，本研究旨在了解小亚热带森林流域溪流顺序（1-5 级）中微生物多样性和群落结构的变化。使用 GIS 软件，选择了 20 条溪流，并将其分为 5 个等级。通过 Illumina 测序来分析微生物群落的动态变化，同时还确定了溪流顺序和溪流水质的水化学性质。我们的结果表明，细菌和真菌的丰富度（ACE 指数）在低等级（1 级和 2 级）溪流中高于高等级（3、4 和 5 级）溪流，其中 2 级溪流的丰富度最高（P<0.05）。水温与溶解氧与真菌丰富度呈正相关（P<0.05）。细菌稀有类群与丰度类群显著相关（P<0.05）。不同等级溪流之间的细菌门（Bacteroidetes、Actinobacteria 和 Chytridiomycota）的相对丰度有显著差异（P<0.05）。使用中性群落模型，我们发现真菌群落结构主要受水化学性质的影响，而细菌群落结构主要受随机过程的调节。我们的研究结果表明，亚热带源头的微生物群落结构的变化主要由水温和溶解氧决定。
