Fujimoto Miyuna, Naiki Yoshikazu, Sakae Kotaro, Iwase Tomohiko, Miwa Naoyoshi, Nagano Keiji, Nawa Hiroyuki, Hasegawa Yoshiaki
Department of Microbiology, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
Department of Pediatric Dentistry, School of Dentistry, Aichi Gakuin University, Nagoya, Japan.
J Oral Microbiol. 2023 May 21;15(1):2215551. doi: 10.1080/20002297.2023.2215551. eCollection 2023.
Mfa1 fimbriae of the periodontal pathogen Porphyromonas gingivalis are responsible for biofilm formation and comprise five proteins: Mfa1-5. Two major genotypes, mfa1 and mfa1, encode major fimbrillin. The mfa1 genotype is further divided into the mfa1 and mfa1 subtypes. The properties of the novel mfa1 remain unclear.
Fimbriae were purified from P. gingivalis strains JI-1 (mfa1), 1439 (mfa1), and Ando (mfa1), and their components and their structures were analyzed. Protein expression and variability in the antigenic specificity of fimbrillins were compared using Coomassie staining and western blotting using polyclonal antibodies against Mfa1, Mfa1, and Mfa1 proteins. Cell surface expression levels of fimbriae were analyzed by filtration enzyme-linked immunosorbent assays.
The composition and structures of the purified Mfa1 fimbriae of 1439 was similar to that of JI-1. However, each Mfa1 protein of differential subtype/genotype was specifically detected by western blotting. Mfa1 fimbriae were expressed in several strains such as 1439, JKG9, B42, 1436, and Kyudai-3. Differential protein expression and antigenic heterogeneities were detected in Mfa2-5 between strains.
Mfa1 fimbriae from the mfa170A and mfa170B genotypes indicated an antigenic difference suggesting the mfa170B, is to be utilized for the novel classification of P. gingivalis.
牙周病原体牙龈卟啉单胞菌的Mfa1菌毛负责生物膜形成，由五种蛋白质组成：Mfa1 - 5。两种主要基因型，mfa1和mfa1，编码主要菌毛蛋白。mfa1基因型进一步分为mfa1和mfa1亚型。新型mfa1的特性尚不清楚。
从牙龈卟啉单胞菌菌株JI - 1（mfa1）、1439（mfa1）和安藤（mfa1）中纯化菌毛，并分析其成分和结构。使用考马斯亮蓝染色和针对Mfa1、Mfa1和Mfa1蛋白的多克隆抗体进行蛋白质印迹，比较菌毛蛋白的蛋白质表达和抗原特异性的变异性。通过过滤酶联免疫吸附测定分析菌毛的细胞表面表达水平。
1439纯化的Mfa1菌毛的组成和结构与JI - 1相似。然而，通过蛋白质印迹可特异性检测到不同亚型/基因型的每种Mfa1蛋白。Mfa1菌毛在1439、JKG9、B42、1436和久大 - 3等几种菌株中表达。在菌株之间的Mfa2 - 5中检测到差异蛋白质表达和抗原异质性。
来自mfa170A和mfa170B基因型的Mfa1菌毛显示出抗原差异，表明mfa170B可用于牙龈卟啉单胞菌的新分类。
Zotero 插件
