[具体物种]和[具体物种]之间[相关基因]和基因簇中的系统发育多样性，作为宿主特异性的一个潜在原因。 （注：由于原文中部分内容缺失具体指代，翻译可能不够完整准确，仅根据现有内容进行了大致翻译。）
Phylogenetic diversity in and gene clusters between and , as a potential cause of host specificity.
作者信息
Fujiwara-Takahashi Kaori, Watanabe Takayasu, Shimogishi Masahiro, Shibasaki Masaki, Umeda Makoto, Izumi Yuichi, Nakagawa Ichiro
机构信息
Department of Periodontology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Both the authors have equally contributed to this article.
出版信息
J Oral Microbiol. 2020 Jun 19;12(1):1775333. doi: 10.1080/20002297.2020.1775333.
BACKGROUND
Periodontopathic bacteria in humans and in animals are phylogenetically close and commonly have FimA and Mfa1 fimbriae. However, little is known about how and are phylogenetically different between and . Here, we examined phylogenetic diversity in their and gene clusters.
METHODS
Twenty strains were isolated from the periodontal pocket of 20 dogs. For their genomic information, along with 64 and 11 genomes, phylogenetic relationship between the genotypes of and was examined. Variability of amino acid sequences was examined in the three-dimensional structure of FimA. The distance between strains was calculated for and genes.
RESULTS
Some genotypes in were close to particular types in . Two types of were classified as 70-kDa and 53-kDa protein-coding . The variable amino acid positions were primarily at the outer part of FimA. The genes encoding the structural proteins and the main component were similarly distant from the reference strain in , but not in .
CONCLUSIONS
The differences in the gene clusters between and may result in their host specificity.
背景
人和动物体内的牙周病原菌在系统发育上相近，通常具有菌毛蛋白A（FimA）和菌毛蛋白1（Mfa1）菌毛。然而，关于人和动物体内的牙周病原菌在系统发育上的差异以及差异方式，我们所知甚少。在此，我们研究了它们的FimA和Mfa1基因簇的系统发育多样性。
方法
从20只犬的牙周袋中分离出20株菌株。结合64株FimA和11株Mfa1的基因组信息，研究了FimA和Mfa1基因型之间的系统发育关系。在菌毛蛋白A的三维结构中检测氨基酸序列的变异性。计算FimA和Mfa1基因的菌株间距离。
结果
一些FimA基因型与Mfa1的特定类型相近。两种类型的Mfa1被归类为编码70 kDa和53 kDa蛋白质的类型。可变氨基酸位置主要在菌毛蛋白A的外部。编码结构蛋白和主要成分的基因与Mfa1中的参考菌株距离相似，但在FimA中并非如此。
结论
FimA和Mfa1基因簇的差异可能导致它们的宿主特异性。
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