Hirakawa Takeshi, Tanno Seia, Ohara Kazuaki
Kirin Central Research Institute, Kirin Holdings Company, Ltd., Fujisawa, Kanagawa, Japan.
Front Plant Sci. 2023 May 8;14:1165646. doi: 10.3389/fpls.2023.1165646. eCollection 2023.
Oxidative stress causes cellular damage and genomic instability through the accumulation of reactive oxygen species (ROS) in plants, resulting in reduced crop production. Chemical priming, which can enhance plant tolerance to environmental stress using functional chemical compounds, is expected to improve agricultural yield in various plants without genetic engineering. In the present study, we revealed that non-proteogenic amino acid N-acetylglutamic acid (NAG) can alleviate oxidative stress damage in (Arabidopsis) and (rice). Exogenous treatment with NAG prevented chlorophyll reduction induced by oxidative stress. The expression levels of and , which are regarded as master transcriptional regulators in response to oxidative stress, increased following NAG treatment. Additionally, Arabidopsis plants treated with NAG showed enhanced levels of histone H4 acetylation at and with the induction of histone acetyltransferase and . The results suggest that NAG could enhance tolerance to oxidative stress through epigenetic modifications and contribute to the improvement of crop production in a wide variety of plants under environmental stress.
氧化应激通过植物体内活性氧（ROS）的积累导致细胞损伤和基因组不稳定，从而降低作物产量。化学引发可以使用功能性化合物增强植物对环境胁迫的耐受性，有望在不进行基因工程的情况下提高各种植物的农业产量。在本研究中，我们发现非蛋白质氨基酸N-乙酰谷氨酸（NAG）可以减轻拟南芥和水稻中的氧化应激损伤。用NAG进行外源处理可防止氧化应激诱导的叶绿素减少。作为应对氧化应激的主要转录调节因子的基因和的表达水平在NAG处理后增加。此外，用NAG处理的拟南芥植株在基因和处的组蛋白H4乙酰化水平随着组蛋白乙酰转移酶和的诱导而增强。结果表明，NAG可以通过表观遗传修饰增强对氧化应激的耐受性，并有助于在环境胁迫下提高多种植物的作物产量。
