Chiang Hui-Yu, Lin Cheng-Huang
Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Rd., Taipei, 10677, Taiwan.
Department of Fine Arts, National Taiwan University, No.1, Shida Rd., Da'an Dist., Taipei, 10645, Taiwan.
Anal Sci. 2023 Sep;39(9):1607-1612. doi: 10.1007/s44211-023-00353-1. Epub 2023 May 24.
The use of an RGB-tracking chart for monitoring the reduction of indigo (color changes) based on the LabVIEW machine vision is demonstrated for the first time. In contrast to a normal analytical chromatographic chart, the time scale is used on the X-axis, but the sum of "RGB-pixels" is used on the Y-axis, instead of "signal intensity". The RGB-tracking chart was obtained from an investigation of the process involved in the reduction of indigo, in which a PC camera was used as a detector and LabVIEW machine vision was simultaneously operated. As a result, when sodium dithionite (NaSO) and yeast were used, respectively, during the indigo-reduction processes, two types of reduction processes were found; the optimized timing for dyeing can be easily determined from the RGB-tracking charts. Furthermore, based on the changes in HSV (hue, saturation, lightness), the use of sodium dithionite provides a higher number of hue and saturation when clothes & fabric were dyed. In contrast to this, a longer time was required for the yeast solution to reach the same high number for hue and saturation. After comparing several series of dyed fabrics, we found that the use of an RGB-tracking chart is indeed a reliable novel tool for measuring color changes that occur during the chemical reactions that are associated with this process.
首次展示了基于LabVIEW机器视觉使用RGB跟踪图表来监测靛蓝还原（颜色变化）的情况。与普通分析色谱图不同，X轴使用时间尺度，而Y轴使用“RGB像素”总和，而非“信号强度”。RGB跟踪图表源自对靛蓝还原过程的研究，该过程中使用PC相机作为检测器并同时运行LabVIEW机器视觉。结果，在靛蓝还原过程中分别使用连二亚硫酸钠（NaSO）和酵母时，发现了两种还原过程；从RGB跟踪图表可轻松确定染色的最佳时机。此外，基于HSV（色调、饱和度、明度）的变化，使用连二亚硫酸钠染色时，衣物和织物的色调和饱和度数量更多。相比之下，酵母溶液达到相同高色调和饱和度数量所需时间更长。在比较了几系列染色织物后，我们发现使用RGB跟踪图表确实是测量此过程相关化学反应中发生的颜色变化的可靠新颖工具。
