Department of Rheumatology, Ningde Municipal Hospital of Ningde Normal University, Ningde, 352100, China.
Department of Pathology, Ningde Municipal Hospital of Ningde Normal University, Ningde, 352100, China.
Cell Mol Biol (Noisy-le-grand). 2023 Feb 28;69(2):150-156. doi: 10.14715/cmb/2023.69.2.25.
This study aimed to study the relationship between the expression levels of inflammatory mediators IL-36β and IL-36R and disease symptoms, laboratory indices and somatic immune function in Systemic Lupus Erythematosus (SLE) of different stages. In this research 70 patients with SLE who were treated in public hospitals from February 2020 to December 2021 were randomly divided into the stable group (n=35) and active group (n=35), and serum IL-36 was measured in the two groups β and IL-36R concentration with the standard curve of Enzyme-Linked Immunosorbent Assay (ELISA) to analyze IL-36β and IL-36R concentrations. 36β and IL-36R concentrations were analyzed in relation to the Disease activity score of systemic lupus erythematosus (SLEDAI), disease duration, typical symptoms of SLE and experimental characteristics. Results showed that the differences in IL-36β and IL-36R concentrations between the stable and active groups overall and for each disease duration group were tiny. There was no significant correlation between serum IL-36β and IL-36R concentrations and SLEDAI scores in stable and active patients, and a negative correlation between them and disease duration. Serum inflammatory mediator IL-36R concentrations were significantly higher in patients with mucosal ulcers and the difference was statistically significant. differences in IL-36β concentrations were statistically significant only for indicators of decreased erythrocyte count and IL-36R concentrations were statistically significant for indicators of decreased erythrocyte count, decreased haemoglobin and decreased lymphocytes The differences were huge and tiny in C4 decline, anti-dsDNA, and urinary routine protein. There was a significant positive correlation between IL-36β and IL-36R concentrations in patients with stable and active SLE, with correlation coefficients of 0.448 and 0.452 respectively. The differences in IL-36β and IL-36R concentrations between the stable and active groups were tiny for patients in the stable and active groups as a whole and for all disease groups. The differences in the number of each inflammatory mediator positive cells in the epidermal stratum corneum and superficial dermis between patients in the stable and active groups were tiny. In conclusion, IL-36β and IL-36R proteins in SLE patients are expressed in immune cells as well as epithelial cells of patients, indicating that these two inflammatory mediators may be one of the early signals that activate the immune system of SLE patients and trigger the immune response of patients; the onset of SLE may be associated with the inflammatory response induced by IL-36β and IL-36R.
本研究旨在探讨不同阶段系统性红斑狼疮（SLE）患者中炎症介质白细胞介素-36β（IL-36β）和白细胞介素-36 受体（IL-36R）表达水平与疾病症状、实验室指标和躯体免疫功能的关系。本研究选取 2020 年 2 月至 2021 年 12 月在公立医院治疗的 70 例 SLE 患者，随机分为稳定组（n=35）和活动组（n=35），采用酶联免疫吸附法（ELISA）标准曲线测量两组血清 IL-36β和 IL-36R 浓度，分析 IL-36β 和 IL-36R 浓度与系统性红斑狼疮疾病活动度评分（SLEDAI）、疾病持续时间、SLE 典型症状和实验特征的关系。结果显示，稳定组和活动组患者的 IL-36β 和 IL-36R 浓度总体差异和各疾病持续时间组差异均较小。稳定和活动患者血清 IL-36β 和 IL-36R 浓度与 SLEDAI 评分无显著相关性，与疾病持续时间呈负相关。黏膜溃疡患者血清炎症介质 IL-36R 浓度显著升高，差异有统计学意义。IL-36β 浓度差异仅在红细胞计数减少指标、IL-36R 浓度在红细胞计数减少、血红蛋白减少和淋巴细胞减少指标上有统计学意义，C4 下降、抗 dsDNA 和尿常规蛋白差异显著且微小。稳定和活动 SLE 患者的 IL-36β 和 IL-36R 浓度之间存在显著正相关，相关系数分别为 0.448 和 0.452。稳定和活动组患者的 IL-36β 和 IL-36R 浓度在总体和所有疾病组中差异较小。稳定和活动组患者表皮角质层和浅层真皮中各炎症介质阳性细胞数差异较小。综上所述，SLE 患者的 IL-36β 和 IL-36R 蛋白在免疫细胞和患者上皮细胞中表达，表明这两种炎症介质可能是激活 SLE 患者免疫系统并引发患者免疫反应的早期信号之一；SLE 的发病可能与 IL-36β 和 IL-36R 引起的炎症反应有关。
