Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538, Bratislava, Slovakia.
Chemical Institute of Clermont-Ferrand (ICCF), UMR6096 CNRS-UCA-Sigma, Clermont-Ferrand, France.
Carbohydr Res. 2023 Jul;529:108852. doi: 10.1016/j.carres.2023.108852. Epub 2023 May 19.
Wild blackthorn berries represent an unexplored area in terms of the characterization of the natural biologically active polysaccharide complexes they contain. The antioxidant active fraction extracted from wild blackthorn fruits by hot water extraction (Hw) was subjected to ion-exchange chromatography and yielded six fractions by successive elution with salts. The purified fractions differed in the content of neutral sugars, uronic acids, proteins and phenolics. About 62% of the applied material was recovered from the column, with a higher yield of the fractions eluted with 0.25 M NaCl. Based on the sugar composition of the eluted fractions, several polysaccharide types were observed. The dominant components of Hw are the fractions eluted with 0.25 M NaCl (∼70%), which represent highly esterified homogalacturonan, containing up to 70-80% of galacturonic acids with a low content of rhamnogalacturonan associated with arabinan, galactan or arabinogalactan side chains, but no phenolics. Further, a dark brown polysaccharide material with a yield of ∼17% and with a high content of phenolic compounds, was eluted with alkali (1.0 M NaOH). It mainly represents an acidic arabinogalactan.
野生黑刺李浆果在其所含天然生物活性多糖复合物的特性方面是一个尚未开发的领域。采用热水提取法（Hw）从野生黑刺李果实中提取的抗氧化活性部分经离子交换层析，用盐连续洗脱得到 6 个部分。纯化的部分在中性糖、糖醛酸、蛋白质和酚类物质的含量上存在差异。约 62%的材料从柱上回收，用 0.25 M NaCl 洗脱的部分产率更高。根据洗脱部分的糖组成，观察到几种多糖类型。Hw 的主要成分是用 0.25 M NaCl 洗脱的部分（约 70%），它们代表高度酯化的同质半乳糖醛酸，含有高达 70-80%的半乳糖醛酸，与阿拉伯半乳聚糖相关的鼠李半乳糖醛酸含量较低，与阿拉伯聚糖、半乳糖聚糖或阿拉伯半乳聚糖侧链相连，但不含酚类物质。此外，用碱（1.0 M NaOH）洗脱得到一种产率约为 17%、酚类化合物含量较高的深棕色多糖物质。它主要代表一种酸性阿拉伯半乳聚糖。
