Department of Zoology, Genetics and Physical Anthropology, University of Santiago de Compostela, Santiago de Compostela, 15872 A Coruña, Spain.
Centro Tecnológico de la Carne de Galicia, Rúa Galicia N° 4, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain; Área de Tecnología de los Alimentos, Facultad de Ciencias de Ourense, Universidad de Vigo, 32004 Ourense, Spain.
Food Chem. 2023 Oct 15;423:136351. doi: 10.1016/j.foodchem.2023.136351. Epub 2023 May 12.
The porcine liver could be used for the extraction of zinc-protoporphyrin (ZnPP) as a natural red meat pigment. During the autolysis process, porcine liver homogenates was incubated at pH 4.8 and 45 °C under anaerobic conditions to obtain insoluble ZnPP. After incubation, the homogenates were readjusted at pH 4.8, and at pH 7.5 before being centrifuged at 5500 × g for 20 min at 4 °C and the resulting supernatant were compared with the obtained at pH 4.8 at the beginning of the incubation. The molecular weight distributions of the porcine liver fractions at both pHs were very similar, however, eight essential amino acids were more abundant in fractions obtained at pH 4.8. Regarding the ORAC assay, porcine liver protein fraction at pH 4.8 showed the highest antioxidant capacity but antihypertensive inhibition was similar for both pHs. Peptides with strong bioactivity potential from aldehyde dehydrogenase, lactoylglutathione lyase, SEC14-like protein 3 and others were identified. The findings have demonstrated the potential of the porcine liver to extract natural pigments and bioactive peptides.
猪肝可用于提取锌原卟啉（ZnPP）作为天然红色肉色素。在自溶过程中，将猪肝匀浆在 pH4.8 和 45°C 下无氧条件下孵育，以获得不溶性 ZnPP。孵育后，将匀浆在 pH4.8 下再次调整，然后在 pH7.5 下离心，在 4°C 下以 5500×g 离心 20 分钟，并与孵育开始时在 pH4.8 下获得的上清液进行比较。两种 pH 值下猪肝级分的分子量分布非常相似，但在 pH4.8 下获得的级分中含有更多的 8 种必需氨基酸。关于 ORAC 测定，pH4.8 的猪肝蛋白级分显示出最高的抗氧化能力，但两种 pH 值的抗高血压抑制作用相似。醛脱氢酶、乳酰谷胱甘肽裂合酶、SEC14 样蛋白 3 等具有强生物活性潜力的肽被鉴定出来。这些发现表明猪肝具有提取天然色素和生物活性肽的潜力。
