Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning, People's Republic of China; Engineering Research Center of Natural Medicine Active Molecule Research & Development, Liaoning, People's Republic of China; Key Laboratory of Natural Bioactive Compounds Discovery & Modification, Shenyang, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China; School of Pharmaceutical Science, Shanxi Medical University, Taiyuan, 030000, People's Republic of China.
Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning, People's Republic of China; Engineering Research Center of Natural Medicine Active Molecule Research & Development, Liaoning, People's Republic of China; Key Laboratory of Natural Bioactive Compounds Discovery & Modification, Shenyang, People's Republic of China; School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, 110016, People's Republic of China.
Phytochemistry. 2023 Aug;212:113725. doi: 10.1016/j.phytochem.2023.113725. Epub 2023 May 22.
Daphnane-type diterpenoids, which are scarce in nature, exhibit potent growth-inhibitory activities against various cancer cells. To identify more daphnane-type diterpenoids, the phytochemical components in the root extracts of Stellera chamaejasme L. were analysed in this study using the Global Natural Products Social platform and the MolNetEnhancer tool. Three undescribed 1α-alkyldaphnane-type diterpenoids (1-3; named stelleradaphnanes A-C) and 15 known analogues were isolated and characterised. The structures of these compounds were determined using ultraviolet and nuclear magnetic resonance spectroscopy. The stereo configurations of the compounds were determined using electronic circular dichroism. Next, the growth-inhibitory activities of isolated compounds against HepG2 and Hep3B cells were examined. Compound 3 exhibited potent growth-inhibitory activities against HepG2 and Hep3B cells with half-maximal inhibitory concentration values of 9.73 and 15.97 μM, respectively. Morphological and staining analyses suggested that compound 3 induced apoptosis in HepG2 and Hep3B cells.
达芙烷型二萜类化合物在自然界中较为稀少，对多种癌细胞具有强烈的生长抑制活性。为了鉴定更多的达芙烷型二萜类化合物，本研究利用全球天然产物社会平台和 MolNetEnhancer 工具分析了瑞香狼毒根提取物中的植物化学成分。分离并鉴定了三种未描述的 1α-烷基达芙烷型二萜类化合物（1-3；命名为瑞香狼毒烷 A-C）和 15 种已知类似物。这些化合物的结构通过紫外和核磁共振光谱确定。通过电子圆二色谱确定了化合物的立体构型。接下来，研究了分离得到的化合物对 HepG2 和 Hep3B 细胞的生长抑制活性。化合物 3 对 HepG2 和 Hep3B 细胞具有较强的生长抑制活性，半数最大抑制浓度（IC50）值分别为 9.73 和 15.97 μM。形态学和染色分析表明，化合物 3 诱导 HepG2 和 Hep3B 细胞凋亡。
