Laboratory of Biosensors, Federal University of Santa Catarina, Campus Universitário Reitor João David Ferreira Lima, Florianópolis, SC, 88040-900, Brazil.
Laboratory of Biosensors, Federal University of Santa Catarina, Campus Universitário Reitor João David Ferreira Lima, Florianópolis, SC, 88040-900, Brazil.
Chemosphere. 2023 Sep;334:139016. doi: 10.1016/j.chemosphere.2023.139016. Epub 2023 May 22.
The monitoring of endocrine disruptors in the environment is one of the main strategies in the investigation of potential risks associated with exposure to these chemicals. Bisphenol A is one of the most prevalent endocrine-disrupting compounds and is prone to leaching out from polycarbonate plastic in both freshwater and marine environments. Additionally, microplastics also can leach out bisphenol A during their fragmentation in the water environment. In the quest for a highly sensitive sensor to determine bisphenol A in different matrices, an innovative bionanocomposite material has been achieved. This material is composed of gold nanoparticles and graphene, and was synthesized using a green approach that utilized guava (Psidium guajava) extract for reduction, stabilization, and dispersion purposes. Transmission electron microscopy images revealed well-spread gold nanoparticles with an average diameter of 31 nm on laminated graphene sheets in the composite material. An electrochemical sensor was developed by depositing the bionanocomposite onto a glassy carbon surface, which displayed remarkable responsiveness towards bisphenol A. Experimental conditions such as the amount of graphene, extract: water ratio of bionanocomposite and pH of the supporting electrolyte were optimized to improve the electrochemical performance. The modified electrode displayed a marked improvement in current responses for the oxidation of bisphenol A as compared to the uncovered glassy carbon electrode. A calibration plot was established for bisphenol A in 0.1 mol L Britton-Robinson buffer (pH 4.0), and the detection limit was determined to equal to 15.0 nmol L. Recovery data from 92 to 109% were obtained in (micro)plastics samples using the electrochemical sensor and were compared with UV-vis spectrometry, demonstrating its successful application with accurate responses.
环境内分泌干扰物的监测是调查与这些化学物质接触相关潜在风险的主要策略之一。双酚 A 是最常见的内分泌干扰化合物之一，容易从聚碳酸酯塑料中浸出，无论是在淡水还是海洋环境中。此外，微塑料在水环境中碎片化时也会浸出双酚 A。为了寻找一种高灵敏度的传感器来测定不同基质中的双酚 A，一种创新的生物纳米复合材料已经被开发出来。这种材料由金纳米粒子和石墨烯组成，是通过一种绿色的方法合成的，该方法利用番石榴（Psidium guajava）提取物来还原、稳定和分散。透射电子显微镜图像显示，复合材料中分层的石墨烯片上有均匀分布的金纳米粒子，平均直径为 31nm。通过将生物纳米复合材料沉积在玻碳表面上，开发了一种电化学传感器，该传感器对双酚 A 表现出显著的响应。实验条件，如石墨烯的用量、生物纳米复合材料中提取物与水的比例以及支持电解质的 pH 值都进行了优化，以提高电化学性能。与未覆盖的玻碳电极相比，修饰电极对双酚 A 的氧化表现出明显改善的电流响应。在 0.1mol L Britton-Robinson 缓冲液（pH 4.0）中建立了双酚 A 的校准曲线，检测限被确定为等于 15.0 nmol L。使用电化学传感器从（微）塑料样品中获得了 92%至 109%的回收率数据，并与紫外-可见光谱法进行了比较，证明了其成功应用和准确的响应。
