慢性 D-半乳糖给药会在大鼠的大脑和心脏中诱导出自然衰老的特征。
Chronic D-galactose administration induces natural aging characteristics, in rat's brain and heart.
机构信息
Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Cardiac Electrophysiology Unit, Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.
Neurophysiology Unit, Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, Thailand.
出版信息
Toxicology. 2023 Jun 15;492:153553. doi: 10.1016/j.tox.2023.153553. Epub 2023 May 22.
We aimed to investigate the effect of chronic D-galactose exposure on the mimicking of natural aging processes based upon the hallmarks of aging. Seven-week-old male Wistar rats (n = 12) were randomly assigned to receive either normal saline solution as a vehicle (n = 6) or 150 mg/kg/day of D-galactose subcutaneously for 28 weeks. Seventeen-month-old rats (n = 6) were also included as the chronologically aged controls. At the end of week 28 of the experiment (when the rats reach 35 weeks old and 24 months old), all rats were sacrificed for brain and heart collection. Our results showed that chronic D-galactose exposure mimicked natural aging characteristics of the brain and the heart in terms of deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, altered intercellular communication, and functional impairment. All of which highlight the potential of D-galactose as a substance for inducing brain and cardiac aging in animal experiments.
我们旨在研究慢性 D-半乳糖暴露对基于衰老标志的自然衰老过程模拟的影响。将 7 周龄雄性 Wistar 大鼠（n=12）随机分为接受生理盐水作为载体（n=6）或皮下注射 150mg/kg/d D-半乳糖 28 周组。还纳入了 17 个月大的大鼠（n=6）作为年龄匹配的对照组。在实验的第 28 周结束时（当大鼠达到 35 周龄和 24 月龄时），所有大鼠均被处死以收集大脑和心脏。我们的结果表明，慢性 D-半乳糖暴露在调节营养感应、线粒体功能障碍、细胞衰老、干细胞衰竭、细胞间通讯改变和功能障碍方面模拟了大脑和心脏的自然衰老特征。所有这些都突出了 D-半乳糖在动物实验中作为诱导大脑和心脏衰老的物质的潜力。
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