Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Veterinary Medical Teaching Hospital, Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan.
J Vet Med Sci. 2023 Jul 1;85(7):743-750. doi: 10.1292/jvms.23-0081. Epub 2023 May 23.
The aims of this study were to evaluate metabolic profiles obtained at -14, 14, and 28 days in milk (DIM), and to identify potential predictive biomarkers of Holstein dairy cows with purulent vaginal discharge (PVD) at 28 DIM. The body condition score (BCS) and hematocrit (Hct) were evaluated, and a metabolic profile test (MPT) was performed at -14, 14, and 28 DIM using serum samples. Cows at 28 DIM were classified using a vaginoscopy and divided into groups of healthy cows (n=89) and cows with PVD (n=31). Albumin (Alb), total cholesterol (TCho), calcium (Ca) and, magnesium (Mg) levels were lower in cows with PVD than in healthy cows at 14 DIM. At 28 DIM, levels of Alb, TCho, Ca, blood urea nitrogen (BUN), Mg, and Hct were lower in cows with PVD. A multivariate stepwise logistic regression analysis showed that higher non-esterified fatty acids (NEFA; odds ratios; OR=4.47; P<0.01), lower Alb (OR=0.07; P<0.01) and lower TCho (OR=0.99; P=0.08) at 14 DIM, and lower Hct (OR=0.83; P=0.05), lower Alb (OR=0.12; P<0.01), and lower BUN (OR=0.74; P=0.02) at 28 DIM were significantly associated with PVD. In conclusion, serum Alb levels was a potential indicator associated with PVD, reflecting dietary protein deficiency preceding disease. Our findings suggest that MPT should be considered to monitor health status during the postpartum period for early diagnosis of PVD.
本研究旨在评估产后第 14、28 天和泌乳第 14 天的代谢谱，并确定产后第 28 天患有化脓性阴道分泌物（PVD）的荷斯坦奶牛的潜在预测生物标志物。评估了体况评分（BCS）和红细胞压积（Hct），并在泌乳第 14、28 天使用血清样本进行了代谢谱测试（MPT）。在泌乳第 28 天，通过阴道镜检查对奶牛进行分类，并将其分为健康奶牛（n=89）和患有 PVD 的奶牛（n=31）两组。在泌乳第 14 天，患有 PVD 的奶牛的白蛋白（Alb）、总胆固醇（TCho）、钙（Ca）和镁（Mg）水平低于健康奶牛。在泌乳第 28 天，患有 PVD 的奶牛的 Alb、TCho、Ca、血尿素氮（BUN）、Mg 和 Hct 水平较低。多变量逐步逻辑回归分析表明，泌乳第 14 天非酯化脂肪酸（NEFA）较高（比值比；OR=4.47；P<0.01）、Alb 较低（OR=0.07；P<0.01）和 TCho 较低（OR=0.99；P=0.08），以及泌乳第 28 天 Hct 较低（OR=0.83；P=0.05）、Alb 较低（OR=0.12；P<0.01）和 BUN 较低（OR=0.74；P=0.02）与 PVD 显著相关。总之，血清 Alb 水平是与 PVD 相关的潜在指标，反映了疾病发生前的饮食蛋白质缺乏。我们的研究结果表明，MPT 应该被考虑用于监测产后期间的健康状况，以实现 PVD 的早期诊断。
