Yantai Institute of China Agricultural University, Yantai, China.
College of Advanced Materials Engineering, Jiaxing Nanhu University, Jiaxing, China.
Water Environ Res. 2023 Jun;95(6):e10896. doi: 10.1002/wer.10896.
This study aimed to determine the best fungi to form the algal-bacterial-fungal symbionts and identify the optimal conditions for the synchronous processing of biogas slurry and biogas. Chlorella vulgaris (C. vulgaris) and endophytic bacteria (S395-2) isolated from it, and four different fungi (Ganoderma lucidum, Pleurotus ostreatus, Pleurotus geesteranus, and Pleurotus corucopiae) were used to form different symbiotic systems. Four different concentrations of GR24 were added to systems to examine the growth characteristics, the content of chlorophyll a (CHL-a), the activity of carbonic anhydrase (CA), the photosynthetic performance, the removal of nutrients, and the biogas purification performance. The results suggested that the growth rate, CA, CHL-a content, and photosynthetic performance of the C. vulgaris-endophytic bacteria-Ganoderma lucidum symbionts were higher than the other three symbiotic systems when 10 M GR24 was added. The highest nutrients/CO removal efficiency 78.36 ± 6.98% for chemical oxygen demand (COD), 81.63 ± 7.35% for total nitrogen (TN), 84.05 ± 7.16% for total phosphorus (TP), and 65.18 ± 6.12% for CO was obtained under the above optimal conditions. This approach will provide a theoretical basis for the selection and optimization of the algal-bacterial-fungal symbionts for biogas slurry and biogas purification. PRACTITIONER POINTS: Algae-bacteria/fungal symbiont presents superior nutrients and CO2 removal capacities. The maximum CO2 removal efficiency was 65.18 ± 6.12%. The removal performance was affected by fungi type.
本研究旨在确定形成藻-菌-菌共生体的最佳真菌，并确定同步处理沼气发酵液和沼气的最佳条件。从其中分离出的普通小球藻（C. vulgaris）和内生细菌（S395-2），以及四种不同的真菌（灵芝、糙皮侧耳、美味侧耳和鲍鱼菇），用于形成不同的共生系统。向系统中添加四种不同浓度的 GR24，以研究生长特性、叶绿素 a（CHL-a）含量、碳酸酐酶（CA）活性、光合作用性能、养分去除和沼气净化性能。结果表明，当添加 10 μM GR24 时，C. vulgaris-内生细菌-灵芝共生体的生长速率、CA、CHL-a 含量和光合作用性能均高于其他三种共生系统。在最佳条件下，化学需氧量（COD）、总氮（TN）、总磷（TP）和 CO 的去除效率分别达到 78.36±6.98%、81.63±7.35%、84.05±7.16%和 65.18±6.12%，达到最高水平。该方法将为沼气发酵液和沼气净化中藻-菌-菌共生体的选择和优化提供理论依据。
