Nutrient removal and biogas upgrade using co-cultivation of Chlorella vulgaris and three different bacteria under various GR24 concentrations by induction with 5-deoxystrigol.

Algae symbiosis technology shows great potential in the synchronous treatment of biogas slurry and biogas, which has promising applications. For improving nutrients and CO removal rates, the present work constructed four microalgal systems: Chlorella vulgaris (C. vulgaris) monoculture, C. vulgaris-Bacillus licheniformis (B. licheniformis), C. vulgaris-activated sludge, and C. vulgaris-endophytic bacteria (S395-2) to simultaneously treat biogas as well as biogas slurry under GR24 and 5DS induction. Our results showed that the C. vulgaris-endophytic bacteria (S395-2) showed optimal growth performance along with photosynthetic activity under the introduction of GR24 (10 M). Under optimal conditions, CO removal efficiency form biogas, together with chemical oxygen demand, total phosphorus and total nitrogen removal efficiencies from biogas slurry reached 67.25 ± 6.71%, 81.75 ± 7.93%, 83.19 ± 8.32%, and 85.17 ± 8.26%, respectively. The addition of symbiotic bacteria isolated from microalgae can promote the growth of C. vulgaris, and the exogenous addition of GR24 and 5DS can strengthen the purification performance of the algae symbiosis to achieve the maximum removal of conventional pollutants and CO.