Department of Environmental Science and Engineering, Fudan University, Shanghai, 200433, People's Republic of China.
School of Advanced Materials and Engineering, Jiaxing Nanhu University, Jiaxing, 314001, People's Republic of China.
World J Microbiol Biotechnol. 2023 Jul 8;39(9):245. doi: 10.1007/s11274-023-03647-8.
Algae symbiosis technology shows great potential in the synchronous treatment of biogas slurry and biogas, which has promising applications. For improving nutrients and CO removal rates, the present work constructed four microalgal systems: Chlorella vulgaris (C. vulgaris) monoculture, C. vulgaris-Bacillus licheniformis (B. licheniformis), C. vulgaris-activated sludge, and C. vulgaris-endophytic bacteria (S395-2) to simultaneously treat biogas as well as biogas slurry under GR24 and 5DS induction. Our results showed that the C. vulgaris-endophytic bacteria (S395-2) showed optimal growth performance along with photosynthetic activity under the introduction of GR24 (10 M). Under optimal conditions, CO removal efficiency form biogas, together with chemical oxygen demand, total phosphorus and total nitrogen removal efficiencies from biogas slurry reached 67.25 ± 6.71%, 81.75 ± 7.93%, 83.19 ± 8.32%, and 85.17 ± 8.26%, respectively. The addition of symbiotic bacteria isolated from microalgae can promote the growth of C. vulgaris, and the exogenous addition of GR24 and 5DS can strengthen the purification performance of the algae symbiosis to achieve the maximum removal of conventional pollutants and CO.
藻菌共生技术在沼气浆同步处理中具有很大的潜力，具有广阔的应用前景。为提高营养物质和 CO 的去除率，本研究构建了四种微藻系统：普通小球藻（C. vulgaris）单培养、C. vulgaris-地衣芽孢杆菌（B. licheniformis）、C. vulgaris-活性污泥和 C. vulgaris-内生细菌（S395-2），在 GR24 和 5DS 诱导下同时处理沼气和沼气浆。结果表明，在 GR24（10 M）的引入下，C. vulgaris-内生细菌（S395-2）表现出最佳的生长性能和光合活性。在最佳条件下，沼气中 CO 的去除效率以及沼气浆中化学需氧量、总磷和总氮的去除效率分别达到 67.25±6.71%、81.75±7.93%、83.19±8.32%和 85.17±8.26%。共生细菌的添加可以促进小球藻的生长，外源添加 GR24 和 5DS 可以增强藻菌共生的净化性能，实现常规污染物和 CO 的最大去除。
