Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life Sciences, Lanzhou University, Lanzhou, China.
School of Life Sciences, Northwest Normal University, Lanzhou, China.
Phytother Res. 2023 Sep;37(9):4102-4116. doi: 10.1002/ptr.7872. Epub 2023 May 24.
Radiation can induce nerve cell damage. Synapse connectivity and functionality are thought to be the essential foundation of all cognitive functions. Therefore, treating and preventing damage to synaptic structure and function is an urgent challenge. Astragaloside IV (AS-IV) is a glycoside extracted from Astragalus membranaceus (Fisch.). Bunge is a widely used traditional Chinese medicine in China with various pharmacological properties, including protective effects on the central nervous system (CNS). In this study, the effect of AS-IV on synapse damage and BDNF/TrkB signaling pathway in radiated C57BL/6 mice with X-rays was investigated. PC12 cells and primary cortical neurons were exposed to UVA in vitro. Open field test and rotarod test were used to observe the effects of AS-IV on the motor and explore the abilities of radiated mice. The pathological changes in the brain were observed by hematoxylin and eosin and Nissl staining. Immunofluorescence analysis was used to detect the synapse damage. The expressions of the BDNF/TrkB pathway and neuroprotection-related molecules were detected by Western blotting and Quantitative-RTPCR, respectively. The results showed that AS-IV could improve the motor and explore abilities of radiated mice, reduce pathological damage to the cortex, enhance neuroprotection functions, and activate BDNF/TrkB pathway. In conclusion, AS-IV could relieve radiation-induced synapse damage, at least partly through the BDNF/TrkB pathway.
辐射会导致神经细胞损伤。突触连接和功能被认为是所有认知功能的重要基础。因此，治疗和预防突触结构和功能的损伤是一个紧迫的挑战。黄芪甲苷（AS-IV）是从黄芪（Fisch.）中提取的一种糖苷。Bunge 是中国广泛使用的传统中药，具有多种药理学特性，包括对中枢神经系统（CNS）的保护作用。在这项研究中，研究了 AS-IV 对 X 射线照射的 C57BL/6 小鼠突触损伤和 BDNF/TrkB 信号通路的影响。PC12 细胞和原代皮质神经元在体外暴露于 UVA 下。旷场试验和旋转棒试验用于观察 AS-IV 对运动的影响，探索辐射小鼠的能力。通过苏木精和伊红及尼氏染色观察脑组织的病理变化。免疫荧光分析用于检测突触损伤。通过 Western blot 和 Quantitative-RTPCR 分别检测 BDNF/TrkB 通路和神经保护相关分子的表达。结果表明，AS-IV 可改善辐射小鼠的运动和探索能力，减轻皮质的病理损伤，增强神经保护功能，并激活 BDNF/TrkB 通路。总之，AS-IV 可缓解辐射诱导的突触损伤，至少部分通过 BDNF/TrkB 通路。
