Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of Chinese Medicine, Changchun 130117, China.
Jilin Ginseng Academy, Key Laboratory of Active Substances and Biological Mechanisms of Ginseng Efficacy, Ministry of Education, Changchun University of Chinese Medicine, Changchun 130117, China.
Phytomedicine. 2023 Jul 25;116:154879. doi: 10.1016/j.phymed.2023.154879. Epub 2023 May 13.
The flavonoids and polysaccharides in Portulaca oleracea L. (PO) have significant antibacterial and antioxidant effects, which can inhibit common bacteria and remove free radicals in the body. However, there was little research on the use of PO to alleviate hyperpigmentation and photoaging damage.
This study was to investigate the anti-photoaging and whitening activity mechanism of polysaccharide of PO (POP) in vitro and in vivo.
In this study, 16 fractions obtained by four enzyme-assisted extraction from PO and their scavenging capabilities against 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals were evaluated. Among these fractions, a polysaccharide fraction (VPOP3) showed the strongest biological activity. VPOP3 was characterized by Fourier-transform infrared spectroscopy, molecular weight (MW), and monosaccharide composition analysis, and the protective effect of VPOP3 on photoaging and hyperpigmentation was researched.
VPOP3 is a low-MW acidic heteropolysaccharide with MW mainly distributed around 0.71KDa, arabinose as its main monosaccharide component. VPOP3 reliably reduced the reactive oxygen species levels in cells and zebrafish and the level of lipid peroxidation in zebrafish. In addition, VPOP3 inhibited UVB-induced apoptotic body formation and apoptosis by downregulating caspase-3 and Bax and upregulating Bcl-2 in mitochondrion-mediated signaling pathways. On the other hand, VPOP3 at high concentrations significantly downregulated the expression of microphthalmia-associated transcription factor, tyrosinase (TYR), and TYR-related protein-1 and TYR-related protein-2 in the melanogenic signaling pathway to achieve a whitening effect.
The above results showed that VPOP3 has superior activities of anti-photoaging and anti-melanogenesis and can be utilized as a safe resource in the manufacture of cosmetics.
马齿苋（Portulaca oleracea L.）中的类黄酮和多糖具有显著的抗菌和抗氧化作用，能抑制常见细菌并清除体内自由基。然而，关于马齿苋多糖（POP）用于缓解色素沉着和光老化损伤的研究较少。
本研究旨在探讨马齿苋多糖（POP）在体外和体内的抗光老化和美白活性机制。
本研究通过四种酶辅助提取从马齿苋中获得 16 个馏分，并评估它们对 2,2-二苯基-1-苦基肼和羟基自由基的清除能力。在这些馏分中，一种多糖馏分（VPOP3）表现出最强的生物活性。VPOP3 的结构通过傅里叶变换红外光谱、分子量（MW）和单糖组成分析进行表征，并研究了 VPOP3 对光老化和色素沉着的保护作用。
VPOP3 是一种低 MW 的酸性杂多糖，MW 主要分布在 0.71KDa 左右，其主要单糖组分为阿拉伯糖。VPOP3 能可靠地降低细胞和斑马鱼中的活性氧水平以及斑马鱼中的脂质过氧化水平。此外，VPOP3 通过下调线粒体介导的信号通路中的 caspase-3 和 Bax 并上调 Bcl-2，抑制 UVB 诱导的凋亡小体形成和凋亡。另一方面，VPOP3 在高浓度时显著下调黑色素生成信号通路中的小眼畸形相关转录因子、酪氨酸酶（TYR）及其相关蛋白-1 和 TYR 相关蛋白-2 的表达，从而达到美白效果。
上述结果表明，VPOP3 具有优异的抗光老化和抗黑色素生成活性，可作为制造化妆品的安全资源。
