School of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui 230009, PR China.
School of Pharmacy, Anhui Medical University, Hefei, Anhui 230031, PR China.
Int J Biol Macromol. 2018 Nov;119:617-623. doi: 10.1016/j.ijbiomac.2018.07.138. Epub 2018 Jul 21.
This paper studied the extraction of polysaccharide from Portulaca oleracea L. (POP) by hot water extraction and ethanol precipitation. Structural properties of the extracted polymers were determined. POP was composed of rhamnose, arabinose and galactose in ratios of 1: 2.34: 3.07 with a molecular weight of 1.55 × 10 Da. The neuroprotective effect of POP on Pb-induced neuronal toxicity was then evaluated in vitro and in vivo test. Treatment with POP markedly increased the survival of PC12 cells and repressed the generation of reactive oxygen species following Pb exposure. In Morris water maze analysis, Pb exposure led to an increase in escape latency and a decrease in platform crossing times of rats in the probe test, which could be attenuated by POP treatment. Additionally, the Pb-induced loss of dendritic spine was recovered after feeding rats with POP at 600 mg/kg/day. These results indicated that Pb-induced cognitive impairments could be inhibited by POP.
本文研究了热水提取和乙醇沉淀法从马齿苋（POP）中提取多糖。测定了提取聚合物的结构特性。POP 由鼠李糖、阿拉伯糖和半乳糖组成，摩尔比为 1:2.34:3.07，分子量为 1.55×10 Da。然后在体外和体内试验中评估了 POP 对 Pb 诱导的神经元毒性的神经保护作用。POP 处理可显著提高 PC12 细胞的存活率，并抑制 Pb 暴露后活性氧的产生。在 Morris 水迷宫分析中，Pb 暴露导致在探测试验中大鼠逃避潜伏期增加和平台穿越次数减少，而 POP 处理可减轻这种情况。此外，喂食 600 mg/kg/d POP 后，Pb 诱导的树突棘丢失得到恢复。这些结果表明，POP 可以抑制 Pb 诱导的认知障碍。
