Department of Chemistry, Faculty of Science, Shahid Bahonar University of Kerman, Kerman 76175-133, Iran.
Environment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman P.O. Box 76318-85356, Iran.
Biosensors (Basel). 2023 Apr 30;13(5):514. doi: 10.3390/bios13050514.
In this work, we prepared a novel electrochemical sensor for the detection of tramadol based on a UiO-66-NH metal-organic framework (UiO-66-NH MOF)/third-generation poly(amidoamine) dendrimer (G3-PAMAM dendrimer) nanocomposite drop-cast onto a glassy carbon electrode (GCE) surface. After the synthesis of the nanocomposite, the functionalization of the UiO-66-NH MOF by G3-PAMAM was confirmed by various techniques including X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), field emission-scanning electron microscopy (FE-SEM), and Fourier transform infrared (FT-IR) spectroscopy. The UiO-66-NH MOF/PAMAM-modified GCE exhibited commendable electrocatalytic performance toward the tramadol oxidation owing to the integration of the UiO-66-NH MOF with the PAMAM dendrimer. According to differential pulse voltammetry (DPV), it was possible to detect tramadol under optimized circumstances in a broad concentration range (0.5 μM-500.0 μM) and a narrow limit of detection (0.2 μM). In addition, the stability, repeatability, and reproducibility of the presented UiO-66-NH MOF/PAMAM/GCE sensor were also studied. The sensor also possessed an acceptable catalytic behavior for the tramadol determination in the co-existence of acetaminophen, with the separated oxidation potential of ΔE = 410 mV. Finally, the UiO-66-NH MOF/PAMAM-modified GCE exhibited satisfactory practical ability in pharmaceutical formulations (tramadol tablets and acetaminophen tablets).
在这项工作中，我们基于 UiO-66-NH 金属有机骨架（UiO-66-NH MOF）/第三代聚（酰胺-胺）树枝状大分子（G3-PAMAM 树枝状大分子）纳米复合材料，制备了一种用于检测曲马多的新型电化学传感器。该纳米复合材料制备完成后，通过 X 射线衍射（XRD）、能谱（EDS）、场发射扫描电子显微镜（FE-SEM）和傅里叶变换红外光谱（FT-IR）等多种技术对 UiO-66-NH MOF 的功能化进行了确认。由于 UiO-66-NH MOF 与 PAMAM 树枝状大分子的结合，UiO-66-NH MOF/PAMAM 修饰的 GCE 对曲马多氧化表现出了出色的电催化性能。根据差分脉冲伏安法（DPV），在优化条件下，可以在较宽的浓度范围（0.5 μM-500.0 μM）和较窄的检测限（0.2 μM）下检测到曲马多。此外，还研究了所提出的 UiO-66-NH MOF/PAMAM/GCE 传感器的稳定性、重复性和重现性。该传感器还具有在对乙酰氨基酚共存下测定曲马多的可接受的催化行为，氧化势分离 ΔE = 410 mV。最后，UiO-66-NH MOF/PAMAM 修饰的 GCE 在药物制剂（曲马多片和对乙酰氨基酚片）中表现出了令人满意的实际应用能力。
