Yao Xin, Shen Jinhui, Liu Qingyan, Fa Huanbao, Yang Mei, Hou Changjun
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, P. R. China.
Anal Methods. 2020 Nov 7;12(41):4967-4976. doi: 10.1039/d0ay01503b. Epub 2020 Oct 2.
In this work, we designed and synthesized a nanocomposite comprising an amine-functionalized metal organic framework (UiO-66-NH), a multiwalled carbon nanotube@reduced graphene oxide nanoribbon (MWCNT@rGONR) and a covalent organic framework (COF) synthesized using melamine and cyanuric acidmonomers via polycondensation (represented by MCA). The UiO-66-NH/MCA/MWCNT@rGONR nanocomposite was used as a sensitive platform for an electrochemical aptasensor to detect kanamycin (kana). Owing to the rich chemical functionality, amino-rich structure and excellent electrochemical activity, the cDNA strands with terminal amino groups can not only anchor over the UiO-66-NH/MCA/MWCNT@rGONR surface but also penetrate into the interior of porous UiO-66-NH/MCA/MWCNT@rGONR networks. The characterization of the UiO-66-NH/MCA/MWCNT@rGONR nanocomposite was performed by scanning electronic microscopy (SEM), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) spectroscopy and X-ray diffraction (XRD). Furthermore, cyclic voltammetry (CV) and square wave voltammetry (SWV) were employed for the electrochemical performance study of this biosensor. The results indicated that the UiO-66-NH/MCA/MWCNT@rGONR nanocomposite exhibited high bioaffinity toward the aptamer and the lowest limit of detection at 13 nM (S/N = 3) within a linearity of the kana concentration of 25-900 nM. In addition, it possessed great repeatability, stability and selectivity and obtained satisfactory recovery results in the real analysis of fish meat and milk, indicating the great potential for analytical measurements in food safety.
在本研究中，我们设计并合成了一种纳米复合材料，其由胺功能化金属有机框架（UiO-66-NH）、多壁碳纳米管@还原氧化石墨烯纳米带（MWCNT@rGONR）以及通过三聚氰胺和氰尿酸单体经缩聚反应合成的共价有机框架（以MCA表示）组成。UiO-66-NH/MCA/MWCNT@rGONR纳米复合材料被用作一种用于检测卡那霉素（kana）的电化学适体传感器的灵敏平台。由于其丰富的化学官能团、富含氨基的结构以及优异的电化学活性，末端带有氨基的cDNA链不仅能够锚定在UiO-66-NH/MCA/MWCNT@rGONR表面，还能渗透到多孔的UiO-66-NH/MCA/MWCNT@rGONR网络内部。通过扫描电子显微镜（SEM）、X射线光电子能谱（XPS）、傅里叶变换红外（FT-IR）光谱和X射线衍射（XRD）对UiO-66-NH/MCA/MWCNT@rGONR纳米复合材料进行了表征。此外，采用循环伏安法（CV）和方波伏安法（SWV）对该生物传感器的电化学性能进行了研究。结果表明，UiO-66-NH/MCA/MWCNT@rGONR纳米复合材料对适体表现出高生物亲和力，在卡那霉素浓度为25 - 900 nM的线性范围内，最低检测限为13 nM（S/N = 3）。此外，它具有良好的重复性、稳定性和选择性，并且在鱼肉和牛奶的实际分析中获得了满意的回收率结果，表明其在食品安全分析测量中具有巨大潜力。
