Center for Nano Bio-Detection, National Chung Cheng University, Chiayi 621301, Taiwan.
Instant NanoBiosensors, Co., Ltd., Taipei 115010, Taiwan.
Biosensors (Basel). 2022 Sep 23;12(10):785. doi: 10.3390/bios12100785.
The global pandemic of COVID-19 has created an unrivalled need for sensitive and rapid point-of-care testing (POCT) methods for the detection of infectious viruses. For the novel coronavirus SARS-CoV-2, the nucleocapsid protein (N-protein) is one of the most abundant structural proteins of the virus and it serves as a useful diagnostic marker for detection. Herein, we report a fiber optic particle plasmon resonance (FOPPR) biosensor which employed a single-stranded DNA (ssDNA) aptamer as the recognition element to detect the SARS-CoV-2 N-protein in 15 min with a limit of detection (LOD) of 2.8 nM, meeting the acceptable LOD of 10 copies/mL set by the WHO target product profile. The sensor chip is a microfluidic chip based on the balance between the gravitational potential and the capillary force to control fluid loading, thus enabling the power-free auto-flowing function. It also has a risk-free self-contained design to avoid the risk of the virus leaking into the environment. These findings demonstrate the potential for designing a low-cost and robust POCT device towards rapid antigen detection for early screening of SARS-CoV-2 and its related mutants.
全球 COVID-19 大流行使得人们前所未有地需要用于检测传染性病毒的灵敏、快速的即时检测（POCT）方法。对于新型冠状病毒 SARS-CoV-2，核衣壳蛋白（N 蛋白）是病毒中最丰富的结构蛋白之一，是一种有用的诊断标志物，可用于检测。在此，我们报告了一种光纤颗粒等离子体共振（FOPPR）生物传感器，它采用单链 DNA（ssDNA）适体作为识别元件，在 15 分钟内检测到 SARS-CoV-2 N 蛋白，检测限（LOD）为 2.8 nM，符合世界卫生组织目标产品概况中规定的可接受的 10 拷贝/mL LOD。传感器芯片是一种微流控芯片，基于控制流体加载的重力势能和毛细作用力之间的平衡，从而实现无电源自流式功能。它还具有无风险的自包含设计，可避免病毒泄漏到环境中的风险。这些发现表明，有可能设计出低成本、稳健的 POCT 设备，用于快速抗原检测，从而对 SARS-CoV-2 及其相关突变体进行早期筛查。
