Department of Biological Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Botany, University of Arid Agriculture, Rawalpindi, Pakistan.
Crit Rev Food Sci Nutr. 2024;64(27):9771-9788. doi: 10.1080/10408398.2023.2216782. Epub 2023 May 26.
Algae are exclusively aquatic photosynthetic organisms that are microscopic or macroscopic, unicellular or multicellular and distributed across the globe. They are a potential source of food, feed, medicine and natural pigments. A variety of natural pigments are available from algae including chlorophyll a, b, c d, phycobiliproteins, carotenes and xanthophylls. The xanthophylls include acyloxyfucoxanthin, alloxanthin, astaxanthin, crocoxanthin, diadinoxanthin, diatoxanthin, fucoxanthin, loroxanthin, monadoxanthin, neoxanthin, nostoxanthin, perdinin, Prasinoxanthin, siphonaxanthin, vaucheriaxanthin, violaxanthin, lutein, zeaxanthin, β-cryptoxanthin, while carotenes include echinenone, α-carotene, β-carotene, γ-carotene, lycopene, phytoene, phytofluene. These pigments have applications as pharmaceuticals and nutraceuticals and in the food industry for beverages and animal feed production. The conventional methods for the extraction of pigments are solid-liquid extraction, liquid-liquid extraction and soxhlet extraction. All these methods are less efficient, time-consuming and have higher solvent consumption. For a standardized extraction of natural pigments from algal biomass advanced procedures are in practice which includes Supercritical fluid extraction, Pressurized liquid extraction, Microwave-assisted extraction, Pulsed electric field, Moderate electric field, Ultrahigh pressure extraction, Ultrasound-assisted extraction, Subcritical dimethyl ether extraction, Enzyme assisted extraction and Natural deep eutectic solvents. In the present review, these methods for pigment extraction from algae are discussed in detail.
藻类是专性水生光合生物,它们可以是微观的或宏观的、单细胞的或多细胞的,分布于全球各地。它们是食物、饲料、药物和天然色素的潜在来源。藻类中有多种天然色素,包括叶绿素 a、b、c、d、藻蓝蛋白、类胡萝卜素和叶黄素。叶黄素包括环氧叶黄素、全反式叶黄素、虾青素、十字孢酮、二氢玉米黄质、硅藻黄素、岩藻黄素、洛洛叶黄素、单加氧叶黄素、新叶黄素、硝石叶黄素、perdinin、原叶绿素 a、硅甲藻黄素、vaucheriaxanthin、 violaxanthin、叶黄素、玉米黄质、β-隐黄质,而类胡萝卜素包括echinenone、α-胡萝卜素、β-胡萝卜素、γ-胡萝卜素、番茄红素、phytoene、phytofluene。这些色素可作为药物和营养保健品应用于食品工业,用于生产饮料和动物饲料。提取色素的传统方法有固液萃取、液液萃取和索氏萃取。所有这些方法效率较低、耗时且溶剂消耗较高。为了从藻类生物质中标准化提取天然色素,目前正在采用先进的方法,包括超临界流体萃取、加压液体萃取、微波辅助萃取、脉冲电场、中电场、超高压力萃取、超声辅助萃取、亚临界二甲醚萃取、酶辅助萃取和天然深共晶溶剂。在本综述中,详细讨论了这些从藻类中提取色素的方法。