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藻类色素提取的先进方法:综述。

Advanced methods of algal pigments extraction: A review.

机构信息

Department of Biological Sciences, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Department of Botany, University of Arid Agriculture, Rawalpindi, Pakistan.

出版信息

Crit Rev Food Sci Nutr. 2024;64(27):9771-9788. doi: 10.1080/10408398.2023.2216782. Epub 2023 May 26.

Abstract

Algae are exclusively aquatic photosynthetic organisms that are microscopic or macroscopic, unicellular or multicellular and distributed across the globe. They are a potential source of food, feed, medicine and natural pigments. A variety of natural pigments are available from algae including chlorophyll a, b, c d, phycobiliproteins, carotenes and xanthophylls. The xanthophylls include acyloxyfucoxanthin, alloxanthin, astaxanthin, crocoxanthin, diadinoxanthin, diatoxanthin, fucoxanthin, loroxanthin, monadoxanthin, neoxanthin, nostoxanthin, perdinin, Prasinoxanthin, siphonaxanthin, vaucheriaxanthin, violaxanthin, lutein, zeaxanthin, β-cryptoxanthin, while carotenes include echinenone, α-carotene, β-carotene, γ-carotene, lycopene, phytoene, phytofluene. These pigments have applications as pharmaceuticals and nutraceuticals and in the food industry for beverages and animal feed production. The conventional methods for the extraction of pigments are solid-liquid extraction, liquid-liquid extraction and soxhlet extraction. All these methods are less efficient, time-consuming and have higher solvent consumption. For a standardized extraction of natural pigments from algal biomass advanced procedures are in practice which includes Supercritical fluid extraction, Pressurized liquid extraction, Microwave-assisted extraction, Pulsed electric field, Moderate electric field, Ultrahigh pressure extraction, Ultrasound-assisted extraction, Subcritical dimethyl ether extraction, Enzyme assisted extraction and Natural deep eutectic solvents. In the present review, these methods for pigment extraction from algae are discussed in detail.

摘要

藻类是专性水生光合生物,它们可以是微观的或宏观的、单细胞的或多细胞的,分布于全球各地。它们是食物、饲料、药物和天然色素的潜在来源。藻类中有多种天然色素,包括叶绿素 a、b、c、d、藻蓝蛋白、类胡萝卜素和叶黄素。叶黄素包括环氧叶黄素、全反式叶黄素、虾青素、十字孢酮、二氢玉米黄质、硅藻黄素、岩藻黄素、洛洛叶黄素、单加氧叶黄素、新叶黄素、硝石叶黄素、perdinin、原叶绿素 a、硅甲藻黄素、vaucheriaxanthin、 violaxanthin、叶黄素、玉米黄质、β-隐黄质,而类胡萝卜素包括echinenone、α-胡萝卜素、β-胡萝卜素、γ-胡萝卜素、番茄红素、phytoene、phytofluene。这些色素可作为药物和营养保健品应用于食品工业,用于生产饮料和动物饲料。提取色素的传统方法有固液萃取、液液萃取和索氏萃取。所有这些方法效率较低、耗时且溶剂消耗较高。为了从藻类生物质中标准化提取天然色素,目前正在采用先进的方法,包括超临界流体萃取、加压液体萃取、微波辅助萃取、脉冲电场、中电场、超高压力萃取、超声辅助萃取、亚临界二甲醚萃取、酶辅助萃取和天然深共晶溶剂。在本综述中,详细讨论了这些从藻类中提取色素的方法。

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