Shen Zi-Qi, Jiang Ji-Hang, Li Chang-Tian, Li Yu, Zhou Li-Wei
Internationally Cooperative Research Center of China for New Germplasm Breading of Edible Mushroom, Ministry of Science and Technology, Changchun 130118, China.
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
J Fungi (Basel). 2023 Apr 23;9(5):505. doi: 10.3390/jof9050505.
, the generic type of belonging to , is a precious medicinal wood-inhabiting macrofungus with high commercial potential. To facilitate the medicinal utilization of this fungal resource, transcriptome sequences are newly generated from . strain MS2. In association with the previously generated genome sequences from the same strain by our lab and all available fungal homologous protein sequences in the UniProtKB/Swiss-Prot Protein Sequence Database, a new methodology was employed for genome assembly and annotation. A total of 13,531 protein-coding genes were identified from the new version of the genome of . strain MS2 with a complete BUSCOs of 92.8%, which indicates a remarkable improvement in the accuracy and completeness of the genome assembly. In general, more genes involved in medicinal functions were annotated compared with the original version of the genome annotation, and most of these genes were also found in the transcriptome data of the currently sampled growth period. Given the above, the current genomic and transcriptomic data provides valuable insights into the evolution and metabolites analysis of . .
属于[具体属名未给出]的[具体菌种名未给出],是一种具有很高商业潜力的珍贵药用木生大型真菌。为促进这种真菌资源的药用利用,新生成了来自[具体菌种名未给出]菌株MS2的转录组序列。结合我们实验室之前从同一菌株生成的基因组序列以及UniProtKB/Swiss-Prot蛋白质序列数据库中所有可用的真菌同源蛋白质序列,采用了一种新方法进行基因组组装和注释。从[具体菌种名未给出]菌株MS2的新版本基因组中总共鉴定出13531个蛋白质编码基因,完整BUSCOs为92.8%,这表明基因组组装的准确性和完整性有了显著提高。总体而言,与原始版本的基因组注释相比,注释出了更多参与药用功能的基因,并且这些基因中的大多数也在当前采样生长阶段的转录组数据中被发现。鉴于上述情况,当前的基因组和转录组数据为[具体菌种名未给出]的进化和代谢物分析提供了有价值的见解。