Liu Tongshu, Zheng Yanli, Wang Litao, Wang Xu, Wang Haiyan, Tian Yongqiang
School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, China.
Front Microbiol. 2025 Mar 5;16:1551436. doi: 10.3389/fmicb.2025.1551436. eCollection 2025.
Surfactins, a class of lipopeptide biosurfactants secreted by plant growth-promoting rhizobacteria (PGPR), have garnered significant attention due to their dual functionality in promoting plant growth and controlling plant diseases. Their potential as biopesticides is underscored by their unique physicochemical properties and biological activities. However, the practical application of surfactin is currently limited by its low yield in natural strains.
This study aimed to optimize the culture conditions for BN, a strain with exceptional biocontrol properties, to enhance its surfactin yield. Critical factors, including nitrogen sources and amino acid supplementation, were systematically investigated to determine their impact on surfactin production.
The study revealed that nitrogen sources and amino acid supplementation were pivotal factors influencing surfactin yield. Compared to the baseline, these factors resulted in a remarkable 5.94-fold increase in surfactin production. Furthermore, a positive correlation was established between surfactin yield and biocontrol efficacy. Enhanced surfactin yield was associated with improved antifungal activity, biofilm formation, and rhizosphere colonization capacity of BN on potato plantlets.
These findings provide novel insights into the practical application of surfactin and establish a scientific foundation for the development of innovative and eco-friendly antifungal agents suitable for agricultural use. The results demonstrate that optimizing culture conditions can significantly enhance surfactin yield and biocontrol efficacy, thereby highlighting the potential for sustainable agricultural practices.
表面活性素是一类由植物促生根际细菌（PGPR）分泌的脂肽生物表面活性剂，因其在促进植物生长和控制植物病害方面的双重功能而备受关注。其独特的物理化学性质和生物活性突出了它们作为生物农药的潜力。然而，表面活性素在天然菌株中的低产量目前限制了其实际应用。
本研究旨在优化具有卓越生物防治特性的菌株BN的培养条件，以提高其表面活性素产量。系统研究了包括氮源和氨基酸添加在内的关键因素，以确定它们对表面活性素生产的影响。
研究表明，氮源和氨基酸添加是影响表面活性素产量的关键因素。与基线相比，这些因素使表面活性素产量显著提高了5.94倍。此外，表面活性素产量与生物防治效果之间建立了正相关关系。表面活性素产量的提高与BN对马铃薯幼苗的抗真菌活性、生物膜形成和根际定殖能力的改善有关。
这些发现为表面活性素的实际应用提供了新的见解，并为开发适用于农业的创新型和生态友好型抗真菌剂奠定了科学基础。结果表明，优化培养条件可以显著提高表面活性素产量和生物防治效果，从而突出了可持续农业实践的潜力。
