Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda Vicent Andrés Estellés, 46100 València, Spain.
Department of Nutrition and Feed Technology, Nofima, 5141 Fyllingsdalen, Norway.
Mar Drugs. 2023 May 11;21(5):294. doi: 10.3390/md21050294.
Information on the bioaccessibility of minerals is essential to consider a food ingredient as a potential mineral fortifier. In this study, the mineral bioaccessibility of protein hydrolysates from salmon () and mackerel () backbones and heads was evaluated. For this purpose, the hydrolysates were submitted to simulated gastrointestinal digestion (INFOGEST method), and the mineral content was analyzed before and after the digestive process. Ca, Mg, P, Fe, Zn, and Se were then determined using an inductively coupled plasma spectrometer mass detector (ICP-MS). The highest bioaccessibility of minerals was found in salmon and mackerel head hydrolysates for Fe (≥100%), followed by Se in salmon backbone hydrolysates (95%). The antioxidant capacity of all protein hydrolysate samples, which was measured by Trolox Equivalent Antioxidant Capacity (TEAC), increased (10-46%) after in vitro digestion. The heavy metals As, Hg, Cd, and Pb were determined (ICP-MS) in the raw hydrolysates to confirm the harmlessness of these products. Except for Cd in mackerel hydrolysates, all toxic elements were below the legislation levels for fish commodities. These results suggest the possibility of using protein hydrolysates from salmon and mackerel backbones and heads for food mineral fortification, as well as the need to verify their safety.
关于矿物质生物可利用性的信息对于将食品成分视为潜在矿物质强化剂至关重要。在这项研究中,评估了来自三文鱼()和鲭鱼()鱼骨和鱼头的蛋白质水解物的矿物质生物可利用性。为此,将水解物进行模拟胃肠道消化(INFOGEST 方法),并在消化过程前后分析矿物质含量。然后使用电感耦合等离子体质谱仪(ICP-MS)测定 Ca、Mg、P、Fe、Zn 和 Se。发现三文鱼和鲭鱼头水解物中铁(≥100%)的矿物质生物可利用性最高,其次是三文鱼鱼骨水解物中的硒(95%)。所有蛋白质水解物样品的抗氧化能力(通过 Trolox 等效抗氧化能力(TEAC)测量)在体外消化后增加(10-46%)。使用 ICP-MS 测定了原始水解物中的重金属 As、Hg、Cd 和 Pb,以确认这些产品的无害性。除了鲭鱼水解物中的 Cd 外,所有有毒元素均低于鱼类商品的法规限量。这些结果表明,从三文鱼和鲭鱼的鱼骨和鱼头中提取的蛋白质水解物有可能用于食品矿物质强化,同时需要验证其安全性。