Department of Biomedical Engineering, Schulich School of Engineering, University of Calgary, 2500 University Drive NW, Calgary, Alberta, Canada T2N 1N4.
Department of Cardiac Sciences, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1.
ACS Appl Mater Interfaces. 2023 Jun 7;15(22):26417-26430. doi: 10.1021/acsami.3c03549. Epub 2023 May 26.
Bacterial nanocellulose (BNC) is a naturally derived hydrogel that has recently paved its way in several biomedical applications. Despite its remarkable tissue-like properties, BNC does not express innate anticoagulant or antimicrobial properties; therefore, appropriate post-modification procedures are required to prevent nonspecific adhesion and enhance the hemocompatibility properties of BNC-based biointerface. Here, we report a new class of flexible, lubricant-infused BNC membranes with superior antithrombotic and antibacterial properties. Using chemical vapor deposition, porous BNC membranes were functionalized with fluorosilane molecules and further impregnated with a fluorocarbon-based lubricant. Compared with unmodified BNC membranes and commercially available poly(tetrafluoroethylene) (PTFE) felts, our developed lubricant-infused BNC samples significantly attenuated plasma and blood clot formation, and prevented bacterial migration, adhesion, and biofilm formation and exhibited superior fat and enzyme repellency properties. Moreover, when subjected to mechanical testing, the lubricant-infused BNC membranes demonstrated a significantly higher tensile strength and greater fatigue resistance when compared with unmodified BNC samples and PTFE felts. Overall, the superior mechanical strength and antithrombotic, antibacterial, and fat/enzyme resistant properties observed in the developed super-repellent BNC-based membranes render their application promising for various biofluid-contacting medical implants and tissue engineering constructs.
细菌纳米纤维素（BNC）是一种天然衍生的水凝胶，最近在多种生物医学应用中得到了应用。尽管 BNC 具有出色的类似组织的特性，但它并不表达固有抗凝或抗菌特性；因此，需要适当的后修饰程序来防止非特异性粘附并提高基于 BNC 的生物界面的血液相容性。在这里，我们报告了一类具有优异抗血栓和抗菌性能的新型柔性、注油 BNC 膜。通过化学气相沉积，多孔 BNC 膜用氟硅烷分子进行功能化，然后进一步用基于氟碳的润滑剂进行浸渍。与未改性的 BNC 膜和市售的聚四氟乙烯（PTFE）毛毡相比，我们开发的注油 BNC 样品显著减弱了血浆和血栓的形成，并防止了细菌迁移、粘附和生物膜形成，表现出优异的脂肪和酶排斥性能。此外，在进行机械测试时，与未改性的 BNC 样品和 PTFE 毛毡相比，注油 BNC 膜表现出更高的拉伸强度和更强的耐疲劳性。总的来说，在开发的超疏水性 BNC 基膜中观察到的优异的机械强度、抗血栓、抗菌和抗脂肪/酶性能，使得它们在各种接触生物流体的医疗植入物和组织工程结构中有很好的应用前景。
