School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada.
Department of Mechanical Engineering, McMaster University, 1280 Main Street West, JHE-308A, Hamilton, Ontario L8S 4L7, Canada.
Lab Chip. 2019 Oct 7;19(19):3228-3237. doi: 10.1039/c9lc00608g. Epub 2019 Aug 30.
Micropatterned biofunctional surfaces provide a wide range of applications in bioengineering. A key characteristic which is sought in these types of bio-interfaces is prevention of non-specific adhesion for enhanced biofunctionality and targeted binding. Lubricant-infused omniphobic coatings have exhibited superior performance in attenuating non-specific adhesion; however, these coatings completely block the surfaces and do not support targeted adhesion or patterning. In this work, we introduce a novel lubricant-infused surface with biofunctional micropatterned domains integrated within an omniphobic layer. This new class of micropatterned lubricant-infused surfaces simultaneously promotes localized and directed binding of desired targets, as well as repellency of undesired species, especially in human whole blood. Furthermore, this modification method is easily translatable to microfluidic devices offering a wider range of applications and improved performance for immunoassays in whole blood and inhibition of clot formation in microfluidic channels. The biofunctional micropatterned lubricant-infused surfaces were created through a bench-top straight forward process by integrating microcontact printing, chemical vapor deposition (CVD) of self-assembled monolayers (SAMs) of fluorosilanes, and further infusion of the SAMs with a bio-compatible fluorocarbon-based lubricant layer. The developed surfaces, patterned with anti-CD34 antibodies, yield enhanced adhesion and controlled localized binding of target biomolecules (e.g. antibodies) and CD34 positive cells (e.g. HUVECs) inside microfluidic devices, outperforming conventional blocking methods (e.g. bovine serum albumin (BSA) or poly(ethylene glycol) (PEG)) in buffer and human whole blood. These surfaces offer a straightforward and effective way to enhance blocking capabilities while preserving the biofunctionality of a micropatterned system in complex biological environments such as whole blood. We anticipate that these micropatterned biofunctional interfaces will find a wide range of applications in microfluidic devices and biosensors for enhanced and localized targeted binding while preventing non-specific adhesion.
微图案生物功能表面在生物工程中有广泛的应用。在这些类型的生物界面中，人们寻求的一个关键特性是防止非特异性黏附，以增强生物功能性和靶向结合。注入润滑剂的全疏涂层在减轻非特异性黏附方面表现出了优异的性能；然而，这些涂层完全阻塞了表面，不支持靶向结合或图案化。在这项工作中，我们引入了一种新型的注入润滑剂的表面，其具有集成在全疏层内的生物功能微图案化域。这种新型的微图案化注入润滑剂表面同时促进了所需靶标的局部和定向结合，以及对不需要的物质的排斥性，特别是在人全血中。此外，这种修饰方法很容易转化为微流控器件，为全血中的免疫分析提供了更广泛的应用和更好的性能，并抑制了微流控通道中的血栓形成。生物功能微图案化注入润滑剂表面是通过整合微接触印刷、氟硅烷自组装单层（SAMs）的化学气相沉积（CVD），以及进一步用生物相容性的全氟碳基润滑剂层注入 SAMs，在台式设备上通过简单的直接工艺来制备的。开发的表面图案化有抗 CD34 抗体，可增强靶生物分子（例如抗体）和 CD34 阳性细胞（例如 HUVEC）在微流控装置内的黏附，并实现受控的局部结合，优于传统的阻断方法（例如牛血清白蛋白（BSA）或聚乙二醇（PEG））在缓冲液和人全血中的效果。这些表面提供了一种简单有效的方法来增强阻断能力，同时在全血等复杂生物环境中保持微图案系统的生物功能性。我们预计，这些微图案化生物功能界面将在微流控装置和生物传感器中得到广泛应用，以增强和局部靶向结合，同时防止非特异性黏附。
