Han Jie, Yan Jun, Li Kang, Lin Bencheng, Lai Wenqing, Bian Liping, Jia Rui, Liu Xiaohua, Xi Zhuge
Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China.
Toxics. 2023 May 7;11(5):441. doi: 10.3390/toxics11050441.
Polystyrene (PS) and di-(2-ethylhexyl) phthalate (DEHP) exist widely in the environment. However, their distribution in organisms remains unclear. We used three sizes (50 nm, 500 nm, and 5 μm) of PS and DEHP to study the distribution and accumulation of PS, DEHP, and mono(2-ethylhexyl) phthalate (MEHP) in mice and nerve cell models (HT22 and BV2 cells) and their potential toxicity. Results showed that PS entered the blood of mice, and the distribution of different particle sizes in different tissues was different. After the combined exposure to PS and DEHP, PS carried DEHP, which significantly increased the DEHP content and MEHP content and the highest content of MEHP was in the brain. With the decrease in PS particle size, the contents of PS, DEHP, and MEHP in the body increased. The levels of inflammatory factors were increased in the serum of the PS or/and DEHP group. In addition, 50 nm polystyrene can carry MEHP into nerve cells. These results suggest for the first time that PS and DEHP combined exposure can induce systemic inflammation, and the brain is an important target organ of PS and DEHP combined exposure. This study may serve as a reference for further evaluation of the neurotoxicity induced by combined exposure to PS and DEHP.
聚苯乙烯（PS）和邻苯二甲酸二（2-乙基己基）酯（DEHP）广泛存在于环境中。然而，它们在生物体中的分布仍不清楚。我们使用三种尺寸（50纳米、500纳米和5微米）的PS和DEHP来研究PS、DEHP和邻苯二甲酸单（2-乙基己基）酯（MEHP）在小鼠和神经细胞模型（HT22和BV2细胞）中的分布、积累及其潜在毒性。结果表明，PS进入小鼠血液，不同粒径在不同组织中的分布不同。PS与DEHP联合暴露后，PS携带DEHP，显著增加了DEHP含量和MEHP含量，且MEHP在脑中的含量最高。随着PS粒径的减小，体内PS、DEHP和MEHP的含量增加。PS或/和DEHP组血清中炎症因子水平升高。此外，50纳米聚苯乙烯可携带MEHP进入神经细胞。这些结果首次表明，PS与DEHP联合暴露可诱导全身炎症，脑是PS与DEHP联合暴露的重要靶器官。本研究可为进一步评估PS与DEHP联合暴露所致神经毒性提供参考。
