Katsburg Miriam, Weingart Christiane, Aubry Etienne, Kershaw Olivia, Kikhney Judith, Kursawe Laura, Lübke-Becker Antina, Moter Annette, Skrodzki Marianne, Kohn Barbara, Fulde Marcus
Institute for Microbiology and Epizootics, Freie Universität Berlin, Robert-von-Ostertagstr. 7, 14163 Berlin, Germany.
Veterinary Centre for Resistance Research (TZR), Freie Universität Berlin, 14163 Berlin, Germany.
Vet Sci. 2023 Apr 25;10(5):314. doi: 10.3390/vetsci10050314.
An 8-year-old male Rhodesian Ridgeback was presented with fever and severe thrombocytopenia. Clinical and laboratory examination, echocardiography, blood culture, and pathohistology revealed evidence of infective endocarditis, ischemic renal infarcts, and septic encephalitis. Treatment was started immediately but the dog's condition worsened, and the dog had to be euthanized. The causative strain was detected by blood culture and MALDI-TOF MS and analyzed using whole-genome sequencing and multilocus sequence typing. Antibiotic susceptibility testing did not detect any resistance. The affected heart valve was analyzed using FISH imaging, which showed a streptococcal biofilm on the heart valve. Bacteria in biofilms are recalcitrant to antibiotic treatment. Early diagnosis could be beneficial to treatment outcome. Treatment of endocarditis could be improved by researching the optimal dosage of antibiotics in conjunction with the use of biofilm-active drugs.
一只8岁的雄性罗得西亚脊背犬出现发热和严重血小板减少症。临床和实验室检查、超声心动图、血培养及病理组织学检查发现了感染性心内膜炎、缺血性肾梗死和化脓性脑炎的证据。立即开始治疗，但犬的病情恶化，不得不实施安乐死。通过血培养和基质辅助激光解吸电离飞行时间质谱检测到致病菌株，并使用全基因组测序和多位点序列分型进行分析。抗生素敏感性测试未检测到任何耐药性。使用荧光原位杂交成像分析受影响的心脏瓣膜，结果显示心脏瓣膜上有链球菌生物膜。生物膜中的细菌对抗生素治疗具有抗性。早期诊断可能有利于治疗结果。通过研究抗生素的最佳剂量并结合使用生物膜活性药物，可改善心内膜炎的治疗。
