Bakács T, Tótpál K, Ringwald G
J Clin Lab Immunol. 1986 Apr;19(4):161-5.
Antibody-dependent monocyte-mediated haemolysis of human group A1B erythrocytes was inhibited by O, Rh/D/-ve unsensitized erythrocytes. Effector cells were sterically isolated to prevent binding, recognition, and subsequent lysis of target cells. Reduction of cytotoxicity was strongest when only B, intermediate when only A1 antigens were saturated, whereas haemolysis could not be significantly inhibited by 200-fold excess of inhibitor cells if both A1 and B antigens were simultaneously saturated. Dilution of anti-A,B serum of between 1:32-1:512 did not reduce cytotoxicity in the absence of inhibitor cells, while inhibition of cytotoxicity grew proportionally stronger by every dilution step in the presence of inhibitor cells. If effector cells were immobilized by adherence, inhibition was strong and could not be influenced by the sensitizing antibody concentration. It was concluded that motility of monocytes was influenced by the number of sensitized antibody binding sites.
O型、Rh/D阴性未致敏红细胞可抑制抗体依赖的单核细胞介导的人A1B血型红细胞溶血。效应细胞在空间上被隔离，以防止与靶细胞结合、识别及随后的裂解。当仅B抗原被饱和时，细胞毒性降低最为显著；当仅A1抗原被饱和时，细胞毒性降低程度中等；而当A1和B抗原同时被饱和时，即使加入200倍过量的抑制细胞，溶血也不能被显著抑制。在没有抑制细胞的情况下，抗A、B血清稀释至1:32 - 1:512不会降低细胞毒性，而在有抑制细胞的情况下，每一步稀释都会使细胞毒性抑制作用成比例增强。如果效应细胞通过黏附固定，抑制作用很强，且不受致敏抗体浓度的影响。由此得出结论，单核细胞的运动性受致敏抗体结合位点数量的影响。
