Escande D, Coulombe A, Faivre J F, Coraboeuf E
J Mol Cell Cardiol. 1986 May;18(5):547-51. doi: 10.1016/s0022-2828(86)80920-8.
The slow inward current isi plays a prominent role in impulse formation and slow conduction in the human heart. For this reason, it may be involved in a great variety of arrhythmias that occur clinically, particularly at the supraventricular level. isi also appears to be the major target of numerous cardioactive drugs daily administered to many patients. Difficulties encountered in voltage clamping human tissues, inevitably dissected from restricted zones of myocardium with inadequate geometry, may explain why direct measurements of this current have never been performed on multicellular human preparations. The recent development of enzymatic techniques allowing the isolation of calcium-tolerant myocytes has provided a solution to these problems. To our knowledge only one abstract has presented until now records of isi in human ventricular myocytes. We describe here the result of experiments carried out to investigate the slow inward current in single cells isolated from adult human atria. The present work provides the first quantitative description of this current in human myocytes.
缓慢内向电流 isi 在人体心脏冲动形成和缓慢传导中起重要作用。因此，它可能与临床上发生的多种心律失常有关，尤其是在室上性心律失常方面。isi 似乎也是每天给予许多患者的众多心脏活性药物的主要作用靶点。在对人体组织进行电压钳制时遇到困难，这些组织不可避免地是从心肌受限区域分离出来的，其几何形状不合适，这可能解释了为什么从未在多细胞人体标本上直接测量过这种电流。酶技术的最新发展使得能够分离出耐钙心肌细胞，为解决这些问题提供了一种方法。据我们所知，到目前为止，只有一篇摘要报道了人体心室肌细胞中 isi 的记录。我们在此描述了为研究从成年人体心房分离的单个细胞中的缓慢内向电流而进行的实验结果。本研究首次对人体心肌细胞中的这种电流进行了定量描述。
